Effects of growth conditions of anther donor plants on the production of pollen plants in wheat triticum aestivum anther culture
Pei, W.; Chen, Y.
Acta Genetica Sinica 7(1): 64-71
1980
Accession: 005306196
Anther cultures of winter wheat (T. aestivum) with anthers taken from F1 hybrid plants grown under 3 different conditions were compared, and significant and reproducible results were obtained. The 3 growth conditions were sowing seed in autumn in the field, transferring the seedlings to the greenhouse in winter, and harvesting anthers for culture in late Feb. and early March the next year; normal autumn-sowing in the field and harvesting anthers for culture in late April the next year; sowing germinated and vernalized winter wheat seed in spring in the field just before the soil ice began to melt and harvesting anthers for culture in the middle of May. The induction frequencies of pollen callus and pollen plants of the anther culture of spring-sown winter wheat were about twice as high as those of the normally autumn-sown winter wheat, and about 3 times as high as those of the greenhouse-grown winter wheat. In 1978, F1 anthers of 28 crosses of spring-sown winter wheat were cultured, in which all the crosses gave rise to pollen callus, and 92.3% gave rise to green pollen plants. The cause of the high induction frequency of the spring-sown winter wheat is analyzed and discussed.