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Effects of linolenic acid on spinach chloroplast structure



Effects of linolenic acid on spinach chloroplast structure



Plant and Cell Physiology 18(3): 551-560



The effects of various concentrations of linolenic acid on the membrane structure of chloroplasts were studied to correlate them with changes in chloroplast capacity to bind free fatty acid. Linolenic acid induced a decrease in absorbance of chloroplasts at 540 nm, especially marked at concentrations between 20 and 60 .mu.M. EM studies revealed that the decrease was a result of the unfolding of grana stacks. Ratios of bound to free linolenic acid also increased markedly in the same range of linolenic acid concentration. Results indicate that linolenic acid-induced changes in chloroplast structure are the main cause of the changes in chloroplast capacity to bind free fatty acid. Linolenic acid-induced structure changes of chloroplasts were reversible. Treatment with 100 .mu.M linolenic acid transformed chloroplasts into assemblies of numerous vesicles. However, addition of bovine serum albumin restored the original configuration of class II chloroplasts, i.e., grana stacks interwoven by intergrana thylakoids. The mode of action of linolenic acid on the chloroplast structure is discussed in terms of hydrophobic association of grana thylakoids.

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Accession: 005312143

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