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Effects of low temperature acclimation of winter rye secale cereale cultivar puma on catalytic properties of its ribulose bis phosphate carboxylase oxygenase


Canadian Journal of Biochemistry 57(7): 1036-1041
Effects of low temperature acclimation of winter rye secale cereale cultivar puma on catalytic properties of its ribulose bis phosphate carboxylase oxygenase
A comparison was made of the kinetics of the carboxylation reaction of bicarbonate Mg-activated ribulose biphosphate carboxylase-oxygenase purified from cold-hardened and unhardened winter rye (S. cereale L. cv. Puma). The activity of the (NH4)2SO4-precipitated enzyme from hardened plants was stable at -20.degree. C for a month, whereas the form from unhardened plants was reversibly cold inactivated. The Km of the unhardened form increased more rapidly with decreasing pH below 8.2, but the estimated pKa of chemical groups associated with the active site was not affected by the cold hardening. The temperature dependencies of the Km CO2 of the 2 forms of the enzyme crossed at 10.degree. C with the effect that the catalysis of carboxylation by ribulose biphosphate carboxylase-oxygenase from Puma rye was most efficient in the temperature range to which the plants were adapted.


Accession: 005312941



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