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Effects of micro tubular inhibitors on cell cycle and conjugation of oxytricha bifaria



Effects of micro tubular inhibitors on cell cycle and conjugation of oxytricha bifaria



Archiv fuer Protistenkunde 125(1-4): 297-308



The role of microtubules was investigated in the conjugation process of the hypotrich ciliate O. bifaria by using colchicine [COL] and vincristine [VCR]. The lowest drug concentrations used (COL: 1.8 mg/ml, VCR: 1.2 .times. 10-2 mg/ml), which proved to be uneffective on the growth rate, partially prevented cell conjugation, while higher dosages (COL: 10 mg/ml, VCR: 0.1 mg/ml) not only totally inhibited cell conjugation but also split early couples. The effects of the 2 inhibitors were completely reversible. The possible mechanism of action of the drugs is considered.

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Accession: 005314359

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