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Effects of micro tubular inhibitors on cell cycle and conjugation of oxytricha bifaria

Archiv fuer Protistenkunde 125(1-4): 297-308

Effects of micro tubular inhibitors on cell cycle and conjugation of oxytricha bifaria

The role of microtubules was investigated in the conjugation process of the hypotrich ciliate O. bifaria by using colchicine [COL] and vincristine [VCR]. The lowest drug concentrations used (COL: 1.8 mg/ml, VCR: 1.2 .times. 10-2 mg/ml), which proved to be uneffective on the growth rate, partially prevented cell conjugation, while higher dosages (COL: 10 mg/ml, VCR: 0.1 mg/ml) not only totally inhibited cell conjugation but also split early couples. The effects of the 2 inhibitors were completely reversible. The possible mechanism of action of the drugs is considered.

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Accession: 005314359

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