Effects of physicochemical treatments on a silkworm densonucleosis virus yamanashi isolate of the silkworm bombyx mori
Applied Entomology and Zoology 21(4): 515-518
ISSN/ISBN: 0003-6862 Accession: 005319780
Effects of physicochemical treatments on the silkworm densonucleosis virus (Yamanashi isolate) (Y-DNV) were investigated. Y-DNV was not inactivated by either sodium desoxycholate or ether. Under 0.5 and 1.0% formalin treatments for 10 min, it was inactivated considerably, but almost completely at 3.0%. pH was not inactivated in the range of pH 3.0-12.0, but was inactivated at pH 2.0 for 1 hr. By ultraviolet irradiation, Y-DNV was noticeably inactivated for 20 min, and completely for 60 min. Y-DNV was not inactivated at 50.degree. C for 60 min, inactivated to some degree at 60.degree. C, but completely at 70.degree. C treatment for 20 min.