Effects of the adrenergic agonists isoprenaline and noradrenaline and the alpha-blocking agents dihydroergotamine and phentolamine on the lipolysis in isolated fat cells of the rat, guinea pig, dog and man

Milavec-Krizman, M.; Wagner, H.

Biochemical Pharmacology 27(19): 2305-2310


ISSN/ISBN: 0006-2952
PMID: 728183
Accession: 005329463

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The actions of noradrenaline (norepinephrine) and isoprenaline and of the .alpha.-adrenoceptor antagonists dihydroergotamine (DHE) and phentolamine, on lipolysis in isolated fat cells from the rat, guinea pig, dog and man were investigated. In isolated rat fat cells, the efficacy of noradrenaline in stimulating lipolysis was equal to that of isoprenaline. In guinea pig, dog and particularly in human fat cells, the maximal effects elicited by noradrenaline were lower than those elicited by isoprenaline. In these tissues the efficacy of noradrenaline was elevated to isoprenaline levels in the presence of low concentrations of DHE or phentolamine. DHE alone produced a weak stimulation of lipolysis in rat fat cells and an intense stimulation in canine fat cells. Phentolamine had no effect. In concentrations of 10-5 M and above DHE and phentolamine inhibited catecholamine stimulated lipolysis in fat cells from all species tested, probably as a result of unspecific effects.