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Electrophoretic studies on poly ploid amphibians part 2 peptidases


, : Electrophoretic studies on poly ploid amphibians part 2 peptidases. Comparative Biochemistry and Physiology B 58(3): 221-226

Starch gel electrophoresis associated with a specific staining method revealed the occurrence of 4 distinct peptidases in diploid and tetraploid Odontophrynus americanus and 3 in O. cultripes. These enzymes can be distinguished on the basis of substrate specificity and electrophoretic mobility. The distribution of the different peptidases in diploid and tetraploid populations of O. americanus and O. cultripes tissues suggests homology among 3 of these peptidases. Mobility, activity and number of peptidases in diploid and tetraploid O. americanus are the same. Peptidase 3 of O. americanus, not detected in O. cultripes, seems to be a gene duplication product which arose after the separation of the evolving lines of the two species, since this peptidase is also detected in the diploid O. americanus. The results show an increase of locus number which, however, cannot be explained as locus duplication of previously duplicated genes by polyploidy. In terms of tissue distribution and substrate specificity, no homology between Odontophrynus and human peptidases could be assumed.

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