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Electrophoretic studies on the cell envelope of a spina producing marine pseudomonad


Canadian Journal of Microbiology 32(12): 901-908
Electrophoretic studies on the cell envelope of a spina producing marine pseudomonad
The effect of physiological parameters known to determine the transition from unspined to spined phenotype in marine pseudomonad D71 has been evaluated using electrophoretic techniques. The levels of at least four protein species (65, 42, 21, and 14 kilodalton (kDa)) in whole-cell lysates were affected. The 65-, 42-, and 14-kDa species were shown to be the major constituents of the outer membrane isolated by gradient separation of spheroplast lysate or detergent dissociation. Both the 42- and 21-kDa species were detected in immunoblots of outer membrane using antispinin antiserum exhaustively preabsorbed with unspined cells. The 21-kDa species was detected in both unspined and spined outer membrane, whereas the 42-kDa species, which migrated with the same mobility as purified spinin, was detected only in spined outer membrane. The 65-kDa species was no longer immunodetected and so likely represented a surface-exposed polypeptide common to both phenotypes. The structure and content of the lipopolysaccharide as determined by electrophoretic analysis was unchanged during the transition in phenotype. Lipopolysaccharide could not be detected in untreated whole-cell lysates, but could be detected chemically and electrophoretically after digestion of the cells. Comparison with LPS species from Salmonella minnesota indicated a deep-rough structure.


Accession: 005349017



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