+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Electrophoretic studies on the cell envelope of a spina producing marine pseudomonad

, : Electrophoretic studies on the cell envelope of a spina producing marine pseudomonad. Canadian Journal of Microbiology 32(12): 901-908

The effect of physiological parameters known to determine the transition from unspined to spined phenotype in marine pseudomonad D71 has been evaluated using electrophoretic techniques. The levels of at least four protein species (65, 42, 21, and 14 kilodalton (kDa)) in whole-cell lysates were affected. The 65-, 42-, and 14-kDa species were shown to be the major constituents of the outer membrane isolated by gradient separation of spheroplast lysate or detergent dissociation. Both the 42- and 21-kDa species were detected in immunoblots of outer membrane using antispinin antiserum exhaustively preabsorbed with unspined cells. The 21-kDa species was detected in both unspined and spined outer membrane, whereas the 42-kDa species, which migrated with the same mobility as purified spinin, was detected only in spined outer membrane. The 65-kDa species was no longer immunodetected and so likely represented a surface-exposed polypeptide common to both phenotypes. The structure and content of the lipopolysaccharide as determined by electrophoretic analysis was unchanged during the transition in phenotype. Lipopolysaccharide could not be detected in untreated whole-cell lysates, but could be detected chemically and electrophoretically after digestion of the cells. Comparison with LPS species from Salmonella minnesota indicated a deep-rough structure.

(PDF 0-2 workdays service)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 005349017

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Anonymous, 1961: CATTLE QUADRUPLETS. An illustrated report is given of quadruplets born to a Marche cow in Recanati. Although birth weights were below average, the calves (2 and 2) were lively and perfectly formed.

Vassoyevich N. B., 1971: The significance of investigating organic material in modern and fossil sediments. Pages 5-11 1971

Adkins A.M.; Knowles R., 1986: Denitrification of cytophaga johnsonae strains and by a gliding bacterium able to reduce nitrous oxide in the presence of acetylene and sulfide. A gliding bacterium (Is-11), isolated for its ability to reduce nitrous oxide (N2O) in the presence of acetylene (C2H2) and sulfide, was able to use nitrate (NO3-), nitrite (NO2-), and N2O as terminal electron acceptors for growth. Similarly, of f...

Thornhill T.S.; Scott R.D., 1989: Unicompartmental total knee arthroplasty. Orthopedic Clinics of North America 20(2): 245-256

Alexander, D. L.; Kern, W., 2005: Drive for show and putt for dough? An analysis of the earnings of PGA Tour golfers. This article examines the determinants of the earnings of PGA Tour golfers from the period 1992-2001. The goal is to determine whether the returns to various golf skills have changed over time. In recent years, golf analysts have claimed that driv...

Ballario, Paolo; Macino, Giuseppe, 1997: White collar proteins PASsing the light signal in Neurospora crassa. Trends in Microbiology 5(11): 458-462, Nov

Singh R.B.; Singh V.P.; Cameron E., 1981: Magnesium in athero sclerotic cardio vascular disease and sudden death. Mg ions are important for maintaining the functional and structural integrity of the [human] myocardium. Epidemiologic studies suggest that myocardial hypomagnecytia can predispose to sudden cardiac death and that the hard water protective factor...

Weiss T.; Lariccia P.J.; Anderson J.; Levy L.; Kinchla J.; Krantz D.S., 1984: Effects of pharmacologic alteration of beta adrenergic activity on electrocardiographic t wave amplitude. Psychophysiology 21(5): 603-604

Haugan, R., 1992: Anzia centrifuga, a new lichen species from Porto Santo, Madeira. Mycotaxon 44(1): 45-50

Bunyatyan A.A.; Flerov E.V.; Tolmachev V.A.; Sablin I.N.; Mierbekov E.M., 1985: Computer assisted monitoring of cerebral bioelectric activity during surgery. Combined computer-assisted monitoring of circulation and central nervous system has been conducted during 42 cardiosurgical operations (closed mitral commissurotomia, correction of valvular diseases under the conditions of hypothermal perfusion)....