+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Electrophoretic study of aged soman inhibited butyrylcholinesterase ec

, : Electrophoretic study of aged soman inhibited butyrylcholinesterase ec Biochimie (Paris) 66(3): 235-250

The following states of purified tetrameric form (C4) of human plasma butyrylcholinesterase were studied by electrophoretic techniques: native, inhibited by soman and by methane sulfonyl fluoride and soman-aged. In order to detect a significant conformational change of the aged cholinesterase as compared to the noninhibited (native) species, enzymes were treated with a set of bifunctional reagents (diimidates) of different chain lengths. After denaturation, the crosslink products were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis. The peak areas of the crosslinked species and the parameters of crosslinkability were calculated from densitometric data, vs. the maximal effective reagent length. The effect of occupancy of the esteratic site by substituted phosphonyl group and by methyl-sulfonyl residue on the binding activity of the anionic site was studied by affinity electrophoresis at varying temperatures with immobilized-procainamide as ligand. Apparent dissociation-constants of the enzyme-ligand complexes were estimated from measurement of mobilities vs. ligand concentration. Corresponding thermodynamic quantities were calculated from Van't Hoff plots and basic thermodynamic equations. The reactivity of aged-cholinesterase with diimidates was similar to that of the native enzyme. Affinity for immobilized-procainamide was slightly lowered in aged and inhibited enzymes as compared to the native and sulfonylated enzymes. As for the ligand-induced isomerization of anionic site (A .fwdarw. B), revealed by affinity electrophoresis, the ligand concentration at the midpoint of transition (.hivin.A = 0.5) was slightly greater for the aged enzyme than for the native one. The dealkylation of soman-cholinesterase conjugate (aging) does not seem to induce structural changes detectable in the crosslinkability of lysyl residues at the subunit interfaces and on the surface of the tetrameric enzyme. The affinity of the anionic site and ligand-induced isomerization process were altered in soman-inhibited and aged enzymes. The occurrence of a weak conformational change of the active center and/or the formation of noncovalent bonds between the methylphosphonyl residue and side chain groups as a result of the dealkylation reaction was suggested.

(PDF 0-2 workdays service)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 005349023

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Wu, D.; Zhang, L-Ming.; Jiang, Y., 2011: Cicatricial pemphigoid in accompany with rheumatoid arthritis: a case report. Chinese Medical Sciences Journal 25(3): 182-184

Don, E.K.; Formella, I.; Badrock, A.P.; Hall, T.E.; Morsch, M.; Hortle, E.; Hogan, A.; Chow, S.; Gwee, S.S.L.; Stoddart, J.J.; Nicholson, G.; Chung, R.; Cole, N.J., 2016: A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous System and Neurodegenerative Disease. NlmCategory="UNASSIGNED">Currently there is a lack in fundamental understanding of disease progression of most neurodegenerative diseases, and, therefore, treatments and preventative measures are limited. Consequently, there is a grea...

Luppova, A. N., 1976: A new termite species of the genus Microcerotermes Silvestri (Isoptera, Termitidae) from Turkmenia. Microcerotermes turkmenicus A. Luppova sp.n. is described.

Dionne, F.T.; Dubé, J.Y.; Frenette, G.; Tremblay, R.R., 1980: Effects of endocrine manipulations on oestrogen binding in cytosols from rat skeletal and perineal muscles. Journal of Endocrinology 85(3): 351-358

Mankowski, J.L.; Spelman, J.P.; Ressetar, H.G.; Strandberg, J.D.; Laterra, J.; Carter, D.L.; Clements, J.E.; Zink, M.C., 1994: Neurovirulent simian immunodeficiency virus replicates productively in endothelial cells of the central nervous system in vivo and in vitro. The perivascular location of human immunodeficiency virus-infected cells suggests that the virus enters the central nervous system (CNS) by traversing the blood-brain barrier (BBB). In this study, the simian immunodeficiency virus (SIV) macaque mo...

CORIPPO.C.; BRIGHENTI.G.M., 1960: Intestinal occlusion caused by ascariasis. Rivista di Patologia E Clinica 15: 523-540

Kumar, T.; Glaser, D.A., 1995: Depth discrimination of a crowded line is better when it is more luminant than the lines crowding it. Observers usually cannot discriminate the relative depth of a crowded feature with respect to crowding features about 2 arc min distant if all the features have the same luminance. However, stereo thresholds significantly less than 20 arc sec are...

Holman, E.Alison.; Silver, R.Cohen., 2011: Health status and health care utilization following collective trauma: a 3-year national study of the 9/11 terrorist attacks in the United States. The September 11, 21 terrorist attacks (9/11) presented a unique opportunity to assess the physical health impact of collective stress in the United States. This study prospectively examined rates of physical ailments and predictors of health care...

Merimsky, E.; Firstater, M., 1978: Ectopic thoracic kidney. British Journal of Urology 50(4): 282-282

Burger, Hans, 1947: Der Kleinstendort beim Eichenanbau. The behavior of oaks (Quercus pedunculata and Q. sessiliflora) planted in 1916 at 2 quite similar localities in Canton Solothurn shows the need for careful selection of seed source to allow for relatively minor site differences (here, mainly the o...