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Electrophoretic study of lipo protein fractions isolated by preparative ultra centrifugation


, : Electrophoretic study of lipo protein fractions isolated by preparative ultra centrifugation. Giornale della Arteriosclerosi 4(2): 103-114

A new methodology for the electrophoretic study of lipoprotein classes, as prepared by ultracentrifugation, is described. Sera from 61 normal and hyperlipidemic subjects were examined. Very low density lipoproteins (VLDL) change their electrophoretic mobility on cellulose acetate after isolation. LDL and high density lipoproteins after isolation migrate with the same mobility as in the total serum. Two abnormal bands are also detectable in many normolipidemic and hyperlipidemic subjects: they probably correspond to the late (slow) pre-.beta. and sinking pre-.beta. lipoprotein.

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Related references

Martin R.A.; D.L.I.lesia F.A., 1980: Rapid preparative scale isolation and quantitation of lipo protein fractions by density gradient ultra centrifugation. Clinical Chemistry 26(7): 1025

Foreman, J.R.; Karlin, J.B.; Edelstein, C.; Juhn, D.J.; Rubenstein, A.H.; Scanu, A.M., 1977: Fractionation of human serum lipo proteins by single spin gradient ultra centrifugation quantification of apo lipo protein b and apo lipo protein a i and lipid components. A sensitive and reproducible method was developed for separation of the major serum lipoproteins from 1 ml or less of human serum by isopycnic density gradient ultracentrifugation. The serum, applied to a step gradient (total volume 12.8 ml), was...

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Viikari J.; Haahti E.; Helela T.T.; Juva K.; Nikkari T., 1969: Fractionation of plasma lipo proteins with preparative inst zonal ultra centrifugation method. Scandinavian Journal of Clinical & Laboratory Investigation 23(1): 85-88

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Gasparotto A.; Manzato E.; Marin R.; Giada F.; Baldo G.; Baritussio A., 1983: A micro method for cholesterol determination of serum lipo protein isolated by zonal ultra centrifugation. A fluorimetric enzymatic micromethod was adopted to measure cholesterol directly in the effluent from the zonal rotor without dialysis and concentration using 0.2 ml samples. Human serum was analyzed for lipoprotein cholesterol content by zonal ul...

Iammarino, R.M.; Humphrey, M.; Antolik, P., 1969: Agar gel lipo protein electrophoresis a correlated study with ultra centrifugation. Clinical Chemistry 15(12): 1218-1229

Rouffy J.; Bakir R.; Chanu B.; Goy Loeper J.; Abou A.; Walti H.; Goussault Y.; Neel D.; Dakkak R.; Dreux C., 1980: Comparative study of total cholesterol high density lipo protein cholesterol and low density lipo protein plus very low density lipo protein cholesterol precipitation by concanavalin a and ultra centrifugation. In 3 populations (37 controls, 59 asymptomatic hyperlipemias and 20 cases of arterial hyperlipemia) the total cholesterol, HDL cholesterol and LDL + VLDL cholesterol and their ratio were determined by 2 methods, precipitation by concanavalin A [co...

Hallinan F.M.; Rose M.; Eagleton M.; Tempany E., 1986: Electrophoretic characterization of human parotid saliva protein fractions isolated by preparative isoelectric focusing. Human parotid saliva proteins fractionated by preparative isoelectric focusing into 3 main fractions have been characterised electrophoretically and immunochemically. The anodic proteins constitute a polymorphic family of collagenase and trypsin s...

Utermann, G.; Albrecht, G.; Steinmetz, A., 1978: Polymorphism of apo lipo protein e part 1 methodological aspects and diagnosis of hyper lipo proteinemia type iii without ultra centrifugation. Two methods for phenotyping apolipiprotein E are compared. One is based on preparation of VLDL [very low density lipoprotein] by conventional ultracentrifugation, whereas the other uses heparin/Mg precipitation of VLDL. The same results were obtai...