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Electrophoretic study of lipo protein fractions isolated by preparative ultra centrifugation


, : Electrophoretic study of lipo protein fractions isolated by preparative ultra centrifugation. Giornale della Arteriosclerosi 4(2): 103-114

A new methodology for the electrophoretic study of lipoprotein classes, as prepared by ultracentrifugation, is described. Sera from 61 normal and hyperlipidemic subjects were examined. Very low density lipoproteins (VLDL) change their electrophoretic mobility on cellulose acetate after isolation. LDL and high density lipoproteins after isolation migrate with the same mobility as in the total serum. Two abnormal bands are also detectable in many normolipidemic and hyperlipidemic subjects: they probably correspond to the late (slow) pre-.beta. and sinking pre-.beta. lipoprotein.

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