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Electrophoretic study of papain fragmentation products of walleye pollock and bovine rhod opsins


, : Electrophoretic study of papain fragmentation products of walleye pollock and bovine rhod opsins. Biokhimiya 44(8): 1472-1477

Fragmentation of wall-eyed pollock and bovine rhodopsins by papain in the photoreceptor membrane was studied by sodium dodecyl sulfate electrophoresis. A scheme of step-wise rhodopsin proteolysis is presented. The MW and localization of the carbohydrate and chromophore components of the fragments formed were determined. The photoreceptor membranes of both rhodopsins apparently contain 3 sites accessible to water environment and are indicative of topographical similarity of the rhodopsins.

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