+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Electrophoretic study on the hemo globin of japanese pond frogs

, : Electrophoretic study on the hemo globin of japanese pond frogs. Scientific Report of the Laboratory for Amphibian Biology Hiroshima University 4: 239-248

The Hb of the following specimens was analyzed by starch gel electrophoresis in order to elucidate biochemically the phylogenic relationship among the 3 forms of Japanese pond frog: Rana nigromaculata, R. brevipoda brevipoda, R. b. porosa and reciprocal hybrids between the former 2 forms at various developmental stages from early tadpole to adult frog; and autotriploids and reciprocal allotriploids of R. nigromaculata and R. b. brevipoda. The Hb pattern of R. nigromaculata remarkably differed from those of R. b. brevipoda and R. b. porosa; there was little difference between those of the 2 ssp. Reciprocal hybrids between R. nigromaculata and R. b. brevipoda showed the same Hb pattern, in which the electrophoretic bands consisted of the total of those of the 2 spp. The Hb of auto- and allotriploid frogs showed electrophoretic patterns which were in accord with the constitution of their genomes. The Hb patterns of R. nigromaculata, R. b. brevipoda and R. b. porosa at the tadpole stage differed from those at the frog stage. The transition from tadpole Hb to frog Hb occurred during metamorphosis. The Hb patterns of reciprocal hybrids at various developmental stages consisted of the total of the electrophoretic bands of the parental species, except those just at the completion of metamorphosis.

Accession: 005349105

Submit PDF Full Text: Here

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Related references

Rochette J.; Righetti P.G.; Bianchi Bosisio A.; Vertongen F.; Schneck G.; Boissel J.P.; Labie D.; Wajcman H., 1984: Immobilized ph gradients and reversed phase high performance liquid chromatography a strategy for characterization of hemo globin variants with electrophoretic mobility identical to that of hemo globin a the case of hemo globin san diego. The preparative aspects of immobilized pH gradients applied to abnormal Hb is described. As shown with the example of Hb San Diego, this method is successful even with as small a difference in pHi as 0.01 pH unit. For characterization of such netu...

Schneider R.G., 1974: Differentiation of electrophoretically similar hemo globins such as hemo globin s hemo globin d hemo globin g and hemo globin p or hemo globin a 2 hemo globin c hemo globin e and hemo globin o by electrophoresis of the globin chains. Clinical Chemistry 20(9): 1111-1115

Shimizu K., 1972: Ontogeny of chicken hemo globin part 1 electrophoretic study of the heterogeneity of hemo globin in development. Development Growth & Differentiation 14(1): 43-55

Brnjas Kraljevic J.; Maricic S.; Pifat G., 1976: Hydration of oxy hemo globin carboxy hemo globin deoxy hemo globin and met hemo globin a proton magnetic relaxation study of protein self association in concentrated solutions. Periodicum Biologorum 78(2): 158

Nishikura, K.; Sugita, Y.; Nagai, M.; Yoneyama, Y., 1975: Ethyl iso cyanide equilibria of hemo globin m iwate hemo globin m boston hemo globin m hyde park hemo globin m saskatoon and hemo globin m milwaukee i in half ferric and fully reduced states. Journal of Biological Chemistry 250(17): 6679-6685

Charlesworth D., 1975: A new hemo globin variant hemo globin f sardinia gamma 75 e 19 iso leucine replaced by threonine found in a family with hemo globin g philadelphia beta chain deficiency and a lepore like hemo globin indistinguishable from hemo globin a 2. Acta Haematologica (basel): 347-355

Sawamura O., 1969: Comparative studies on the disc electrophoretic patterns of some erythrocyte components in the new born and adult part 2 electrophoretic heterogeneity of hemo globin a 1 and hemo globin a 2 in the adult blood. Journal of Kyushu Hematological Society 19(4): 225-229

Adachi K.; Asakura T., 1981: Gelation and crystallization of sickle hemo globin s and hemo globin charlem and nonsickle hemo globin hemo globin a and hemo globin c in concentrated phosphate buffer. Brewer, G J (Ed ) Progress in Clinical And Biological Research, Vol 55 The Red Cell; 5th International Conference, Ann Arbor, Mich , Usa, Sept 29-Oct 2, 1980 Xxxiii+806p Alan R Liss, Inc : New York, N Y , Usa Illus P123-142

Matsui, M.; Satow, T., 1977: An electrophoretic analysis of the hemo globin of japanese toads. An electrophoretic analysis of the Hb of Japanese toads, Bufo bufo japonicus, B. b. formosus and B. torrenticola was made from the taxonomic point of view. B. torrenticola had 1 Hb component, irrespective of age and sex, whereas B. b. formosus had...

Gardiner M.B.; Carver J.; Abraham B.L.; Wilson J.B.; Huisman T.H.J., 1982: Further studies on the quantitation of the hemo globin a hemo globin s hemo globin c and hemo globin f in new born babies with different hemo globinopathies using high pressure liquid chromatography. High pressure liquid chromatography (HPLC) was used for the detection and quantitation of the .beta. chain variants Hb S and Hb C in blood samples of newborn babies with different hemoglobinopathies. The complete separation of the Hb C, S, A and F...