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Electrophoretic transfer of proteins from fixed and stained gels

Analytical Biochemistry 141(2): 409-412
Electrophoretic transfer of proteins from fixed and stained gels
A procedure by which sodium dodecyl sulfate [SDS] gels can be fixed and stained with Coomassie blue and subsequently transferred to nitrocellulose for immunostaining is outlined. The procedure involves the complete removal of the stain followed by equilibration of the gel in SDS running buffer. The efficiency of transfer is comparable to unfixed gels and the protein pattern of the transfer appears to be sharper, presumably due to less diffusion during the transfer process. The procedure does not affect the antigenicity of the proteins that were examined by subsequent immunostaining. This method is particularly useful for situations in which sample size or concentration are limiting factors resulting in insufficient material for duplicate gels. Human surfactant protein was used in this study.

Accession: 005349118

PMID: 6208814

DOI: 10.1016/0003-2697(84)90062-9

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