EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Electrophoretic variants of alanine amino peptidase ec 3.4.11.2 alkaline phosphatase ec 3.1.3.1 and gamma glutamyl transferase ec 2.3.2.2 in urine


Clinical Chemistry 30(6): 856-859
Electrophoretic variants of alanine amino peptidase ec 3.4.11.2 alkaline phosphatase ec 3.1.3.1 and gamma glutamyl transferase ec 2.3.2.2 in urine
The 3 brush-border enzymes, alanine aminopeptidase, alkaline phosphatase and .gamma.-glutamyltransferase, are present in the urine of healthy persons in 2 variants, a particulate form and a soluble one. They can be separated by electrophoresis in agarose gel and by ultracentrifugation. The particulate forms exhibit similar electrophoretic mobility, but the soluble forms of these brush-border enzymes differ in their electrophoretic mobilities. The enzyme components of the particulate activity can be mobilized by Triton X-100 and trypsin. The electrophoretic mobility of the soluble forms of alanine aminopeptidase and .gamma.-glutamyltransferase is slowed by neuraminidase treatment. Both forms of .gamma.-glutamyltransferase are influenced in their electrophoretic mobility by treatment with n-butanol/diisopropyl ether, showing their lipid dependence. These findings enhance knowledge of the biochemical nature of brush-border enzymes in urine.


Accession: 005349141



Related references

Serum enzyme alterations during diabetic keto acidosis prospective study of the behavior of alkaline phosphatase aspartate amino transferase alanine amino transferase and gamma glutamyl trans peptidase in 24 cases. Medicina Clinica 77(5): 213-215, 1981

Serum aspartate amino transferase alanine amino transferase alkaline phosphatase lactate dehydrogenase glutamyl trans peptidase and creatine kinase determined in the cobas bio centrifugal analyzer by the methods of the scandinavian committee on enzymes. Scandinavian Journal of Clinical & Laboratory Investigation 42(2): 173-176, 1982

Comparative study of alanine-amino-peptidase and gamma-glutamyl-transferase activity in normal Wistar rat urine. Laboratory Animals 17(3): 246-251, 1983

Electrophoretic variants of alanine aminopeptidase, alkaline phosphatase, and gamma-glutamyltransferase in urine. Clinical Chemistry 30(6): 856-859, 1984

Electrophoretic separation of alkaline phosphatase ec 3.1.3.1 iso enzymes compared with alkaline phosphatase and gamma glutamyl transferase ec 2.3.2.2 in hepato biliary diseases. Scandinavian Journal of Gastroenterology 16(7): 885-890, 1981

Change of aspartate amino transferase alanine amino transferase leucine aryl amidase and gamma glutamyl trans peptidase in epileptic children during anti convulsive treatment. Acta Universitatis Carolinae Medica Monographia (75): 196-197, 1977

Gamma glutamyl trans peptidase leucine amino peptidase and alkaline phosphatase. Schweizerische Medizinische Wochenschrift 99(16): 606-608, 1969

Serum and urinary gamma glutamyl trans peptidase leucine amino peptidase and alkaline phosphatase in the diagnosis of neoplasias. Quaderni Sclavo de Diagnostica Clinica e di Laboratorio 9(1): 374-397, 1973

Part 2 behavior of reactions of alkaline phosphatase gamma glutamyl trans peptidase and amino transferase in the small intestine mucosa of rats poisoned with ethyl alcohol a biochemical and histochemical study. Patologia Polska 29(3): 335-346, 1978

Comparison between the serum levels of gamma glutamyl trans peptidase alkaline phosphatase and leucine amino peptidase in liver diseases. Annali dell'Istituto Superiore di Sanita 7(2-3): 400, 1971