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Electrophoretic variation among and within strains of kharkof wheat maintained at 11 locations


, : Electrophoretic variation among and within strains of kharkof wheat maintained at 11 locations. Euphytica 36(3): 815-822

The hard red winter wheat (Triticum aestivum L.) cultivar Kharkof was introduced to the United States at the turn of the century and has been included as the long-term check for the Southern and Northern Regional Performance Nurseries since 1930. Seed of Kharkof is typically maintained at each site at which the nurseries are grown. We obtained samples of Kharkof from 10 sites and the USDA Small Grains Collection in Beltsville, MD. Up to 54 individual kernels per strain were ground and evaluated for gliadin electrophoretic patterns. Strains from Beltsville, MD and Aberdeen, ID were completely uniform for gliadin patterns; they differed from each other and from all other strains. Seven strains were highly polymorphic, with 7 to 14 gliadin patterns occurring in each. However, there were large differences in pattern frequencies among strains, with the Ft. Collins, CO and Columbia, MO strains at one extreme, the Manhattan, KS and Lincoln, NE strains at the other, and the St. Paul, MN; York, NE; and Clovis, NM strains intermediate. Strains from Dallas and Chillicothe, TX were completely uniform for a pattern that also occurred in all of the polymorphic strains. The two Texas strains also had esterase and .beta.-amylase isoelectric focusing patterns different from the others, and the Beltsville strain had a different .beta.-amylase pattern. We concluded that the strains from Beltsville and Aberdeen were not Kharkof, that the Texas strains were derived from a single-plant selection out of Kharkof, and that the remaining strains have diverged through natural selection possibly operating in conjunction with genetic drift, outcrossing, and seed mixtures.

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Accession: 005349154

DOI: 10.1007/bf00051865

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