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Electrophoretic variations in brassica with respect to esterase iso enzyme patterns

Journal of the Indian Botanical Society 57(2): 146-154

Electrophoretic variations in brassica with respect to esterase iso enzyme patterns

The different stages of germination (0, 24, 48, 72, 96 and 170 h of germination) of 24 genotypes of Brassica (6 genotypes of each of brown sarson, yellow sarson and toria, belonging to B. campestris, and 6 genotypes of rai, belonging to B. juncea) were studied for esterase isoenzyme patterns. The total number of isoenzyme bands increased in the early stages (at 24-h stage in brown sarson and yellow sarson and at 48-h stage in toria and rai) followed by the reduction (except in a few genotypes) in the later stages of germination by showing their differential appearances and disappearances. Such developmental alterations were used in identifying the genotypes because each genotype (except in a few genotypes of rai) had its characteristic pattern of presence, absence, disappearance and reappearance of bands at one or the other stage of germination. Early and medium maturing genotypes were not distinguished from each other but could be distinguished from late maturing genotypes at 0-h stage in yellow sarson, at 0- and 24-h stages in brown sarson, at 24-h stage in toria and at 170 h stage in rai. At 0-h stage of all the genotypes of B. campestris the intraspecific esterase isoenzyme variation was less compared to the interspecific variation.

Accession: 005349195

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