+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Electrophysiological analysis of neurogenic vasodilatation in the isolated lingual artery of the rabbit

British Journal of Pharmacology 89(1): 163-172
Electrophysiological analysis of neurogenic vasodilatation in the isolated lingual artery of the rabbit
1 The nature of neurogenic vasodilatation was investigated in isolated segments of rabbit lingual artery. In separate experiments membrane responses to nerve stimulation were studied by use of microelectrodes. 2 In the presence of guanethidine to block constrictor responses and noradrenaline to induce tone, field stimulation with trains of pulses (8 Hz for 0.5 to 4 s) produced vasodilatation. Atropine (10-6 M) reduced the relaxations to about 50% of the control values while the induced vasodilatations were potentiated by physostigmine. Tetrodotoxin (TTX, 10-7 M) blocked all nerve-evoked responses. These data suggest that there is a cholinergic and a non-cholinergic component of the vasodilatation produced by nerve stimulation in the rabbit lingual artery. 3 Single stimuli did not evoke electrophysiological responses. With parameters similar to those used in the mechanical studies, periarterial stimulation in the presence of guanethidine evoked membrane hyperpolarization which achieved amplitudes of up to 11 mV. The ionophoretic application of acetylcholine (ACh) produced hyperpolarization. 4 The inhibitory junction potentials ( but not the ionophoretic-induced responses were blocked by TTX. The nerve-evoked and the ACh-induced hyperpolarizations were potentiated by physostigmine (5 .times. 10-7 M) and totally blocked by atropine (10-7 M). 5 and hyperpolarization to ionophoresis of ACh were recorded from arteries in which the endothelium had been removed by mechanical rubbing. Mechanical relaxation to field stimulation and ACh was observed in preparations without endothelium. 6 These data suggest that the cholinergic component of the neurogenic vasodilatation in the rabbit lingual artery is accompanied by hyperpolarization. The non-cholinergic component does not appear to possess an electorphysiological correlate. In addition, it seems that the action of nerve-released ACh is mediated by muscarinic receptors which are situated directly on the vascular smooth muscle cells.

Accession: 005349398

PMID: 3801771

DOI: 10.1111/j.1476-5381.1986.tb11132.x

Download PDF Full Text: Electrophysiological analysis of neurogenic vasodilatation in the isolated lingual artery of the rabbit

Related references

Electrophysiological analysis of cholinergic vasodilatation in the isolated rabbit lingual artery. Journal of Physiology (Cambridge) 372: 75P, 1986

Neurogenic vasodilatation in isolated perfused segments of rabbit jejunal artery. Clinical and Experimental Pharmacology & Physiology 20(5): 355-358, 1993

Nitric oxide-mediated neurogenic vasodilatation in isolated monkey lingual arteries. American Journal of Physiology 272(4 Pt 2): H1582-8, 1997

Neurogenic vasodilatation of the rat mesenteric resistance artery i pharmacological analysis. Japanese Journal of Pharmacology 46(SUPPL): 96P, 1988

Calcitonin gene-related peptide mediated neurogenic vasorelaxation in the isolated canine lingual artery. Japanese Journal of Pharmacology 67(4): 329-339, 1995

Inhibition by hydroxyl radicals of calcitonin gene-related peptide-mediated neurogenic vasorelaxation in isolated canine lingual artery. Journal of Pharmacology and Experimental Therapeutics 280(1): 492-500, 1997

Mechanism of vasodilatation induced by substance P in isolated rabbit renal artery. Korean Journal of Veterinary Research 43(4): 573-578, 2003

Atracurium induced vasodilatation is not mediated by histamine in the isolated femoral artery of the rabbit. European Journal Of Anaesthesiology. 13(4): 333-339, 1996

A mechanism underlying histamine-induced contraction in isolated rabbit lingual artery. Nihon Yakurigaku Zasshi. Folia Pharmacologica Japonica 103(4): 175-186, 1994

Influences of the endothelium and hypoxia on neurogenic transmission in the isolated pulmonary artery of the rabbit. British Journal Of Pharmacology. 108(1): 150-154, 1993