EurekaMag.com logo
+ Site Statistics
References:
53,214,146
Abstracts:
29,074,682
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Electrophysiological effects of propionate and bicarbonate on gallbladder epithelium



Electrophysiological effects of propionate and bicarbonate on gallbladder epithelium



American Journal of Physiology 248(1 Pt 1): C58-C69



The effects of propionate and HCO3- on cell membrane potentials in Necturus gallbladder epithelium were investigated using microelectrode techniques in vitro. Bilateral exposure to either propionate or HCO3- (25 mM each) hyperpolarized both cell membranes by about 12 mV. Mucosal addition of either substance produced cyclic changes in voltage of both cell membranes, which attenuated spontaneously, whereas serosal addition caused sustained hyperpolarization. By intraepithelial cable analysis it was shown that both cell membrane conductances rose during the hyperpolarization. Experiments using substitution of mucosal K+ for Na+ revealed that the relative K+ permeability (PK) of the apical membrane was enhanced during the hyperpolarization induced by mucosal (or serosal) propionate (or HCO3-). These effects are mediated by increases in PK at both membranes, with a larger basolateral effect. We suggest that this mechanism accounts for the higher cell membrane potential values measured in epithelia bathed in HCO3--or propionate-containing solutions. Inasmuch as both propionate and HCO3- stimulate fluid absorption in gallbladder epithelium, the increase in cell membrane PK may represent an adaptive response of the cells to regulate their solute content.

(PDF 0-2 workdays service: $29.90)

Accession: 005349594

Download citation: RISBibTeXText

PMID: 2981476



Related references

Effects of cyclic amp on transcellular bicarbonate transport in rabbit gallbladder epithelium. Pfluegers Archiv European Journal of Physiology 408(SUPPL 1): R34, 1987

Electrophysiological effects of basolateral [Na+] in Necturus gallbladder epithelium. Journal of General Physiology 99(2): 241-262, 1992

Electrophysiological effects of extracellular ATP on Necturus gallbladder epithelium. Journal of General Physiology 97(5): 949-971, 1991

Transcellular bicarbonate transport in rabbit gallbladder epithelium: mechanisms and effects of cyclic AMP. Pflugers Archiv 416(3): 312-321, 1990

Side specific effects of carbonic acid and propionate on potassium permeability in necturus gallbladder epithelium. Federation Proceedings 43(4): ABSTRACT 3551, 1984

Electrophysiological effects of mucosal Cl- removal in Necturus gallbladder epithelium. American Journal of Physiology 257(3 Pt 1): C568-C578, 1989

Electrophysiological effects of mucosal chloride removal in necturus gallbladder epithelium. American Journal of Physiology 257(3 PART 1): C568-C578, 1989

Electrophysiological effects of basolateral sodium concentration in necturus gallbladder epithelium. Journal of General Physiology 99(2): 241-262, 1992

Effects of putative chlorine channel blockers on electroneutral and electrogenic bicarbonate secretion by guinea pig gallbladder epithelium in vitro. Journal of Physiology (Cambridge) 418: 22P, 1989

Transport of propionate across the distal colonic epithelium of guinea pig in the presence and absence of bicarbonate and of chloride. Journal of Veterinary Medicine Series A 44(2): 73-78, 1997

Electroneutral secretion of bicarbonate by guinea pig gallbladder epithelium. American Journal of Physiology 250(4 Pt 1): C617-C628, 1986

Propionate induced changes in cell membrane potassium permeability in gallbladder epithelium. Federation Proceedings 41(5): ABSTRACT 7753, 1982

Effects of bicarbonate on fluid and electrolyte transport by the guinea pig gallbladder: a bicarbonate-chloride exchange. Journal of Membrane Biology 45(1-2): 43-59, 1979

Role of chloride in bicarbonate transport across guinea pig gallbladder epithelium. Naunyn-Schmiedeberg's Archives of Pharmacology 329(SUPPL): R42, 1985