+ Translate

Electrophysiological methods in study of the epithelia

, : Electrophysiological methods in study of the epithelia. Folia Medica Cracoviensia 25(3-4): 265-274

The paper includes the review of the techniques used for determining electrophysiological parameters such as: potential difference (PD) and resistance (R) in investigating transporting functions of biological membranes. This study can apply to isolated epithelial models (transepithelial measurements), as well as to cell membrane and even to apical and basolateral parts of epithelial cell membrane (measurements by microelectrodes). The next advantage which follows from using electrophysiological techniques is possibility of application of ionselective microelectrodes for ion concentration determinations. It allows a continous observation of effects of the drugs influencing the transporting action. Measurements of PD and R obtained by micropuncture technique give possibilities of: getting information of cell membrane passive permeability for ions, finding sodium-coupled transport mechanism, and also localizing ion active transport pumps.

(PDF 0-2 workdays service)

Accession: 005349704

Submit PDF Full Text: Here

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Related references

Rae, James L., 1992: Patch clamp methods for the study of ocular epithelia. Experimental Eye Research 55(SUPPL 1): S96

Karnaky, K.J.; Jr., 1992: Electrophysiological assessment of epithelia. Unknown

Miani, P.; Piragine, F., 1962: Studies on the vibratile epithelia of the upper airpassages. Note I. Methods used for the study of ciliary movement. La Clinica Otorinolaringoiatrica 14: 143-171

Schultz, S.G.; Hudson, R.L.; Lapointe, J.Y., 1985: Electrophysiological studies of sodium cotransport in epithelia: toward a cellular model. During the past two decades, microelectrophysiological studies of small intestine and renal proximal tubule employing conventional as well as ion-selective microelectrodes have contributed significantly to our understanding of the nature of Na-cou...

Lapointe J Y., 1986: Electrophysiological studies of sodium cotransport in epithelia toward a cellular model. Semenza, G. Kinne (ed.). Annals Of The New York Academy Of Sciences, Vol. 456. Membrane Transport Driven By Ion Gradients; Meeting, New York, N.y., Usa, Oct. 1-4, . Xii 462p. The New York Academy Of Sciences: New York, N.y., Usa. Illus. 127-135, 1985

Brayden, D.; Moriarty, D.; Baird, A., 1997: A novel in vitro electrophysiological bioassay for transport of loperamide across intestinal epithelia. Pharmaceutical Research (new York). 14(7): 942-945

Scragg, M.A.; Johnson, N.W., 1980: A comparison of in vivo and in vitro stathmokinetic methods for the study of cell proliferation in hamster oral epithelia. Mitotic indices (MI) expressed as numbers of metaphase fingers per 100 basal cells in the cheek pouch and palatal epithelium of the Syrian hamster following metaphase arrest with vinblastine sulphate (VLB) were compared using in vivo and in vitro...

Van Driessche, W.; Simaels, J.; Aelvoet, I.; Erlij, D., 1988: Cation-selective channels in amphibian epithelia: electrophysiological properties and activation. 1. Na+ as well as Li+ move across the apical membrane through amiloride-sensitive ionic channels. 2. K+ movements across the apical membrane occur through Ba2+- and Cs+-sensitive channels which do not allow the passage of Na+ or Li+. 3. A third pa...

Nardi J.B.; Norby S W.; Magee Adams S.M., 1987: Cellular events within peripodial epithelia that accompany evagination of manduca wing discs conversion of cuboidal epithelia to columnar epithelia. In the wing disc of Manduca, a sheet of peripodial epithelium completely covers the apical surface of another epithelium destined to form the wing blade. The cubodial cells of the peripodial epithelium not only are attached to a thick basal lamina...

Inoue, I., 1993: Electrophysiological methods fro study of macromolecular systems. Tanpakushitsu Kakusan Koso. Protein, Nucleic Acid, Enzyme 38(7): 1297-1302