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Electrophysiological methods in study of the epithelia

, : Electrophysiological methods in study of the epithelia. Folia Medica Cracoviensia 25(3-4): 265-274

The paper includes the review of the techniques used for determining electrophysiological parameters such as: potential difference (PD) and resistance (R) in investigating transporting functions of biological membranes. This study can apply to isolated epithelial models (transepithelial measurements), as well as to cell membrane and even to apical and basolateral parts of epithelial cell membrane (measurements by microelectrodes). The next advantage which follows from using electrophysiological techniques is possibility of application of ionselective microelectrodes for ion concentration determinations. It allows a continous observation of effects of the drugs influencing the transporting action. Measurements of PD and R obtained by micropuncture technique give possibilities of: getting information of cell membrane passive permeability for ions, finding sodium-coupled transport mechanism, and also localizing ion active transport pumps.

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