EurekaMag.com logo
+ Site Statistics
References:
53,214,146
Abstracts:
29,074,682
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Electrophysiological properties of cultured dorsal root ganglion and spinal cord neurons of normal and trisomy 16 fetal mice






Developmental Brain Research 32(1): 111-122

Electrophysiological properties of cultured dorsal root ganglion and spinal cord neurons of normal and trisomy 16 fetal mice

Dorsal root ganglion (DRG) and spinal cord neurons from normal and trisomy 16 fetal mice, and animal model for human trisomy 21 (Down syndrome), were maintained in primary culture and their electrical membrane properties were compared with intracellular recording techniques. After 3-4 weeks in culture, trisome DRG neurons had a higher mean resting potential (+10%), a higher specific membrane resistance (+50%) and higher excitability (+17%), a shorter action potential (-22%), higher maximal rates of depolarization (+39%) and of two phases of repolarization (+20% and +10%) and a lower duration (-42%) of the afterhyperpolarization, than did control DRG neurons (P < 0.05). The duration of the action potential was 2 .times. greater than in control neurons, when external calcium was elevated from 1.2 to 10 mM. Differences in the electrical parameter like those observed in DRG neurons also were found in cultured spinal cord neurons. These results indicate that trisomy 16 in fetal mice alters passive and active electrical membrane properties in DRG and spinal cord neurons, and suggest that some differences are related to differences in calcium currents.

Accession: 005349728

PMID: 2952222

DOI: 10.1016/0165-3806(87)90144-1

Download PDF Full Text: Electrophysiological properties of cultured dorsal root ganglion and spinal cord neurons of normal and trisomy 16 fetal mice



Related references

Electrical properties of cultured dorsal root ganglion neurons from normal and trisomy 21 human fetal tissue. Brain Research 474(2): 246-254, 1988

Effects of nerve growth factor on whole-cell currents and other electrical membrane properties in cultured dorsal root ganglion neurons from normal and trisomy 16 mice. Brain Research. 650(1): 161-165, 1994

Single sodium fluctuations in cultured normal and trisomy 16 dorsal root ganglion drg neurons from the fetal mouse. Society for Neuroscience Abstracts 13(3): 1600, 1987

Voltage-activated sodium conductances in cultured normal and trisomy 16 dorsal root ganglion neurons from the fetal mouse. Brain Research 466(2): 265-274, 1988

Calbindin D-28K and calretinin immunoreactive neurons in trisomy 13 mice A study of developmental spinal cord and dorsal root ganglion. Neuroscience Research Supplement 0(18): S116, 1993

Ontogenesis of calbindin D-28K in the dorsal root ganglia and spinal cord of normal and trisomy 13 fetal mice. Experientia (Basel) 49(ABSTR ): A71, 1993

Mouse fetal trisomy 13 and hypotrophy of the spinal cord: Effect of calbindin-D-28k and calretinin expressed by neurons of the spinal cord and dorsal root ganglia. Neuroscience 57(4): 1109-1120, 1993

Effect of theophylline upon electrophysiological properties of cultured mouse dorsal root ganglion neurons. Polish Journal of Pharmacology & Pharmacy 44(SUPPL ): 113, 1992

Electrical membrane properties of cultured dorsal root ganglion neurons from trisomy 19 mouse fetuses: a comparison with the trisomy 16 mouse fetus, a model for Down syndrome. Brain Research 511(1): 169-172, 1990

Calcium currents and transmitter output in cultured spinal cord and dorsal root ganglion neurons. Journal of Neurophysiology 56(5): 1257-1267, 1986