EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Electrophysiological studies in principal cells of rat cortical collecting tubules adh increases the apical membrane sodium conductance


, : Electrophysiological studies in principal cells of rat cortical collecting tubules adh increases the apical membrane sodium conductance. Pfluegers Archiv European Journal of Physiology 409(1-2): 81-92

The mechanism of ion transport across principal cells of rat cortical collecting tubules (CCT) and its regulation by vasopressin (ADH) has been studied in the isolated perfused tubule. To amplify the response to ADH rats were treated with 5 mg I. M. desoxycorticosterone 4-9 days prior to the experiments. Addition of 2 .cntdot. 10-10 mol .cntdot. l-1 ADH increased the transepithelial voltage from -5.1 .+-. 0.7 mV to -16.1 .+-. 1.4 mV (n = 37) and decreased the transepithelial resistance from 51 .+-. 4 .OMEGA. cm2 to 39 .+-. 2 .OMEGA. cm2 (n = 33). Optical and functional differentiation of impalements of principal and intercalated cells was made and only data of principal cells are presented. ADH depolarized the apical membrane from 79 .+-. 1 mV to 66 .+-. 2 mV (n = 26) and decreased the fractional resistance of the apical membrane from 0.76 .+-. 0.04 to 0.70 .+-. 0.04 (n = 13). These ADH effects were prevented by 10-5 or 10-4 mol .cntdot. l-1 luminal amiloride which hyperpolarized the apical membrane when added in the presence or absence of ADH. Apical and basolateral membranes were dominated by large K+ conductances and addition of 3 mmol .cntdot. l-1 barium to bath or lumen perfusates increased transepithelial resistance almot two-fold, whereas luminal amiloride increased the transepithelial resistance only by 26-35%. Ouabain (0.5 mmol .cntdot. l-1, bath) depolarized the basolateral membrane and decreased its K+ conductance. These effects were prevented by the simultaneous presence of apical amiloride suggesting that the only route of Na+ entry into the principal cells occurred via the amiloride sensitive Na+ conductance. We conclude that ADH stimulates Na+ reabsorption and K+ secretion in the rat CCT primarily by increasing the Na+ conductance in the apical cell membrane.

(PDF 0-2 workdays service)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 005349768

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Comandini, E; Zardi, U., 1983: Revamping ammonia and urea plants: big returns for a small investment. Fertilizer 83 International Conference: proceedings of the conference London 13-16 November 1983 editor AI More: 441

Borzyak E.I., 1970: Contribution to anatomy of superior and inferior tracheo bronchial lymph nodes. Arkhiv Anatomii Gistologii i Embriologii 59(12): 54-63

Pager, Cara Theresia; Wurth, Mark Allen; Dutch, Rebecca Ellis, 2003: Proteolytic cleavage of the Hendra virus fusion protein. Hendra virus (HeV) is an emerging paramyxovirus. The HeV fusion protein precursor is proteolytically cleaved into the disulfide-linked subunits, F1 and F2. An unusual cleavage site and the propagation of virus in a furin-negative cell line, sugges...

Brown, D.; Luttrell, C.; Casson, A.; Cruz, R.; Fomete, T., 2004: Forest law enforcement & governance: the role of independent monitors in the control of forest crime. Illegal logging is an issue of major national and international concern. Combating illegal logging depends on effective enforcement operations to ensure compliance and identify forest crime. Independent monitors have an important role in 'mon...

Tokarev V.; Gostin V., 2005: Mount Lofty Ranges, South Australia. Pages 214-219 2005

Sen D.N., 1972: Survival adaptations of vegetation in dry environment. Indian Science Congress Association Proceedings 59(PART 3): 392

Kope, S., 1999: Video camera drape. Official Gazette of the United States Patent & Trademark Office Patents 1220(3): 2335, March 16

Catherall P.L.; Boulton R.E., 1979: Reaction of some winter oat cultivars to oat mosaic virus and oat tubular virus. In 4 of 5 seasons in Wales [UK] during 1970-1976, the winter oat cultivars Maris Osprey, and Peniarth were very susceptible to oat mosaic virus, Padarn was moderately susceptible and Pendrwm and Century were resistant. Plants of resistant cultivar...

Drury, Ln; Mitchell, Bw, 1981: Solar poultry brooding system performance with microcomputer control strategy versus conventional control. Paper American Society of Agricultural Engineers Microfiche collection: (fiche no 81-4556)

Bann Kerrie L.; Gingras Murray K.; MacEachern James A., 2004: Changes in latitude, changes in attitude; two outstanding modern occurrences of the Teredolites ichnofacies. Teredolites ichnocoenoses are examined from the modern temperate bay-margin of Willapa Bay, Washington, USA and subtropical Moreton Bay of Queensland, Australia. In particular, in situ log-grounds from the upper intertidal zones are considered. Th...