EurekaMag.com logo
+ Translate

Electrophysiological study of conditioning lesion effects of rat sciatic nerve regeneration following either prior section or freeze ii. blocking by prior tenotomy


, : Electrophysiological study of conditioning lesion effects of rat sciatic nerve regeneration following either prior section or freeze ii. blocking by prior tenotomy. Brain Research 449(1-2): 150-156

The conditioning lesion effects refer to the earlier formation and the accelerated regenration of axonal sprouts following two successive axotomines. In a previous study, we observed that a prior freeze or a prior cut of rat sciatic nerve resulted in differences in the enhancement of the regeneration rate and the reduction of the initial delay. These differences were interpreted as possible non-neuronal cells influence the intrinsic regulation of the conditioning lesion phenomenon. In the present study, we attempted to modify the status of the muscles using tenotomy before the prior nerve injury to determine the respective influence of the muscular cells on conditioning lesion effects. Thus, the conditioning lesion, which was either a cut or a freeze of the tibial nerve at the ankle, was performed 14 days after foot sole muscles were tenotomized, close to their insertion into the calcaneus bone. The test lesion was always a freeze of the sciatic nerve at midthigh performed 7 days following the prior lesion. The elongation of the regenerating sprouts was electrophysiologically evaluated and the regeneration rate as well as the initial delay were calculated by means of regression analysis. Tenotomy did not influence the regeneration as was demonstrated in a group with a single sciatic nerve lesion. In contrast, when prior lesion was performed, the tenotomy prevented both the enhancement of the rate of regeneration and the reduction of the initial delay, whatever was the type of the conditioning lesion. Since tenotomy is known to reduce protein synthesis in muscular cells, the ability of the tenotomized muscles to maintain a supply of trophic survival factors to the motor neuron could be reduced. On the other hand, if the conditioning lesion effects require some denervation-induced muscular factors promoting axonal outgrowth, their amount following tenotomy would be lesser. In both cases, subsequent axotomy would fail to elicit normal cell body response, therefore preventing the conditioning lesion effects.

(PDF 0-2 workdays service)

Accession: 005349832

PMID: 3395843

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Bondoux-Jahan, M.; Sebille, A., 1988: Electrophysiological study of conditioning lesion effects on rat sciatic nerve regeneration following either prior section or freeze. II. Blocking by prior tenotomy. The conditioning lesion effects refer to the earlier formation and the accelerated regeneration of axonal sprouts following two successive axotomies. In a previous study, we observed that a prior freeze or a prior cut of rat sciatic nerve resulted...

Bondoux-Jahan, M.; Sebille, A., 1986: Electrophysiological study of conditioning lesion effect on rat sciatic nerve regeneration following either prior section or freeze. I. Intensity and time course. A peripheral nerve lesion performed distally prior to a proximal axotomy is known to result in an increase in the rate of regeneration of both sensory and motor fibres. This phenomenon is called the 'conditioning lesion effect'. The aim...

Perry G.W.; Krayanek S.R.; Wilson D.L., 1987: Effects of a conditioning lesion on bullfrog sciatic nerve regeneration analysis of fast axonally transported proteins. We have shown that bullfrog sciatic nerves respond to a conditioning lesion similarly to goldfish optic nerve and rat or mouse sciatic nerve; that is, following a crush the rate of regeneration is faster in nerves that have received a conditioning...

Bondoux Jahan M.; Sebille A., 1989: Conditioning lesion effects on rat sciatic nerve regeneration are influenced by electrical stimulation delivered to denervated muscles. The rate of regeneration and the initial delay of the fastest growing fibers of the rat sciatic nerve were electrophysiologically evaluated after a freeze at mid-thigh. A prior section or a prior freeze at the ankle level increased the rate of reg...

Ryoke, K.; Ochi, M.; Iwata, A.; Uchio, Y.; Yamamoto, S.; Yamaguchi, H., 2000: A conditioning lesion promotes in vivo nerve regeneration in the contralateral sciatic nerve of rats. A conditioning lesion in the sciatic nerve increases in vivo axonal regeneration in the nerve after a second transection. We studied whether this increased regeneration also occurs in the contralateral nerve. The left sciatic nerve was transected...

Mcquarrie, I.G.; Grafstein, B.; Dreyfus, C.F.; Gershon, M.D., 1978: Regeneration of adrenergic axons in rat sciatic nerve effect of a conditioning lesion. The rat sciatic nerve was crushed at the level of the mid-thigh (testing lesion), and the rate of outgrowth of the regenerating adrenergic axons was examined in the nerve. Two methods were used: counts of axons containing norepinephrine demonstrab...

McQuarrie, I.G.; Grafstein, B.; Gershon, M.D., 1977: Axonal regeneration in the rat sciatic nerve: effect of a conditioning lesion and of dbcAMP. After the sciatic nerve had been crushed at the level of the mid-thigh, the rate of outgrowth of the regenerating axons was measured by using the pinch test to locate the leading sensory axons. This standard crush lesion ("testing" lesio...

Ruigt, G.S.F.; Den Brok Bardoel, M., 1993: The pinch test as a model for studying regeneration of rat sural and tibial nerves following sciatic nerve crushing or freezing Effects of a conditioning lesion, vincristine, cisplatin, IGF-1 and isaxonin. Society for Neuroscience Abstracts 19(1-3): 423

Abdullaeva, G.M.; Gamiiants, S.A., 1988: Regeneration of the sciatic nerve in the progeny of female rabbits irradiated prior to mating. A study was made of the sciatic nerve regeneration after smooth cutting thereof in 198 young chinchillas whose mothers were subjected to whole-body single irradiation with doses of 0.05, 0.15, 0.5 or 1.5 Gy one week before mating. The statisticall...

Gordon, H.S.; Zeleny Pooley, M.; Wang, M.S.; Gold, B.G., 1998: Efficacy of delayed or discontinuous FK506 administration on nerve regeneration in the sciatic nerve crush model Lack of evidence for a conditioning lesion-like effect. Society for Neuroscience Abstracts 24(1-2): 813