EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Electrophysiology of oocytes during meiotic maturation and after ovulation in brook trout salvelinus fontinalis


, : Electrophysiology of oocytes during meiotic maturation and after ovulation in brook trout salvelinus fontinalis. Canadian Journal of Zoology 63(8): 1904-1908

Brook trout oocytes were stripped of their follicular layers and were impaled with both voltage-measuring and current-passing microelectrodes. The membrane resting potential (Vm) was -46 .+-. 1.2 mV (mean .+-. SE, n = 50, bath grounded) in control denuded oocytes with an intact germinal vesicle. Vm was reduced to -38 .+-. 1.8 mV (n = 16) in eggs that had ovulated in vivo. Accompanying the potential drop was a marked increase in membrane resistance (Rm) from 26 .+-. 1.7 in denuded oocytes to 1640 .+-. 150 k.OMEGA. .cntdot. cm2 in ovulated eggs and a reduction in membrane current (Im) from 2380 .+-. 260 to 25 .+-. 2.6 nA .cntdot. cm-2. The increased resistance in ovulated eggs was in part the result of reduced Na+ and K+ membrane conductances; there was no significant change in Cl- conductance. Treatment of denuded oocytes with 17.alpha.-20.beta.-dihydroxyprogesterone produced a germinal vesicle dissolution; in denuded oocytes that had undergone germinal vesicle dissolution in vitro, Rm increased and Im decreased, comparable at least qualitatively to the changes seen after ovulation in vivo. The results indicate that major electrophysiological changes accompany germinal vesicle dissolution and ovulation in brook trout oocytes.

(PDF same-day service: $19.90)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 005349904

DOI: 10.1139/z85-283

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Antunes, J. L.; Zimmerman, E. A., 1978: The hypothalamic magno cellular system of the rhesus monkey an immuno cytochemical study. The hypothalamic magnocellular system of the adult female rhesus monkey was studied using immunoperoxidase technique and antisera to estrogen stimulated neurophysin (ESN), nicotine stimulated neurophysin (NSN), oxytocin (OT) and vasopressin (VP)....

Anonymous, 2010: East Asian Seas Time-series EAST-I. Ocean and Polar Research 32.3

Gobel, Erwin J. A. M.; Hautvast, Raymond W. M.; Van Gilst, Wiek H.; Spanjaard, Jan N.; Hillege, Hans L.; Dejongste, Mike J. L.; Molhoek, G. Peter; Lie, Kong I., 1995: Randomised, double-blind trial of intravenous diltiazem versus glyceryl trinitrate for unstable angina pectoris. The effect of dihydropyridines in patients with unstable angina is discouraging. To find out the effect of the non-dihydropyridine-like calcium-channel blocker diltiazem, a randomised, double-blind trial was conducted comparing diltiazem with glyc...

Von Konow L.; Nord C.E., 1983: Ornidazole compared to phenoxymethyl penicillin in the treatment of oro facial infections. A double blind, randomized study comprising 60 patients was undertaken to compare the efficacy of ornidazole to that of phenoxymethylpenicillin in the treatment of orofacial infections. Thirty patients received ornidazole 500 mg orally every 12 h...

Kubitza, Dagmar; Becka, Michael; Wensing, Georg; Voith, Barbara; Zuehlsdorf, Michael, 2005: Safety, pharmacodynamics, and pharmacokinetics of BAY 59-7939 - an oral, direct Factor Xa inhibitor - after multiple dosing in healthy male subjects. Objectives: There is a clinical need for safe new oral anticoagulants. The safety, tolerability, pharmacodynamics, and pharmacokinetics of BAY 59-7939 - a novel, oral, direct Factor Xa (FXa) inhibitor - were investigated in this single-center, pla...

Prince, Homer E.; Painter, T. S.; Morrow, Marie B.; Meyer, George H., 1961: Studies with dust extracts. Extracts prepared from house dust reacted with greater frequency on patients sensitive to house dust than did extracts of dusts from other sources, including a department store, dormitories, hotels and an office. Extracts of dust from old feather...

Dury, A., 1962: Effect of reserpine on fatty acids mobilization. Fed Proc: 284

Castanon-Najera, Guillermo; Latournerie-Moreno, Luis, 2004: Comportamiento de familias S-1 de maiz en distintos pH del suelo. This research was carried out in three environments of Vera Cruz State, Mexico, during Spring-Summer 1997. The objectives of the work were to evaluate the response of S-1 families at environments with and Without soil acidity, to estimate genetic...

Rayns, David G., 1974: Collagen from frozen fractured glycerinated beef heart. Journal of Ultrastructure Research 48(1): 59-66

Zunzunegui, M.; Diaz Barradas, M. C.; Aguilar, F.; Ain Lhout, F.; Clavijo, A.; Garcia Novo, F., 2002: Growth response of Halimium halimifolium at four sites with different soil water availability regimes in two contrasted hydrological cycles. The relationship between water availability and plant growth response in Halimium halimifolium (L.) Willk throughout two years with contrasted precipitation (300 and 850 mm) was examined by measuring vegetative growth and midday leaf water potenti...