EurekaMag.com logo
+ Site Statistics
References:
53,214,146
Abstracts:
29,074,682
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Electrophysiology of oocytes during meiotic maturation and after ovulation in brook trout salvelinus fontinalis






Canadian Journal of Zoology 63(8): 1904-1908

Electrophysiology of oocytes during meiotic maturation and after ovulation in brook trout salvelinus fontinalis

Brook trout oocytes were stripped of their follicular layers and were impaled with both voltage-measuring and current-passing microelectrodes. The membrane resting potential (Vm) was -46 .+-. 1.2 mV (mean .+-. SE, n = 50, bath grounded) in control denuded oocytes with an intact germinal vesicle. Vm was reduced to -38 .+-. 1.8 mV (n = 16) in eggs that had ovulated in vivo. Accompanying the potential drop was a marked increase in membrane resistance (Rm) from 26 .+-. 1.7 in denuded oocytes to 1640 .+-. 150 k.OMEGA. .cntdot. cm2 in ovulated eggs and a reduction in membrane current (Im) from 2380 .+-. 260 to 25 .+-. 2.6 nA .cntdot. cm-2. The increased resistance in ovulated eggs was in part the result of reduced Na+ and K+ membrane conductances; there was no significant change in Cl- conductance. Treatment of denuded oocytes with 17.alpha.-20.beta.-dihydroxyprogesterone produced a germinal vesicle dissolution; in denuded oocytes that had undergone germinal vesicle dissolution in vitro, Rm increased and Im decreased, comparable at least qualitatively to the changes seen after ovulation in vivo. The results indicate that major electrophysiological changes accompany germinal vesicle dissolution and ovulation in brook trout oocytes.

(PDF same-day service: $19.90)

Accession: 005349904

DOI: 10.1139/z85-283



Related references

Hormonal changes during meiotic maturation and ovulation in the brook trout (Salvelinus fontinalis). Fish Physiology and Biochemistry 3(4): 203-211, 1987

The effects of steroids on final maturation and ovulation of oocytes from brook trout (Salvelinus fontinalis) and yellow perch (Perca flavescens). Biology of Reproduction 18(2): 293-298, 1978

Effects of primaquine and incubation ph on the in vitro ovulation of brook trout salvelinus fontinalis oocytes. American Zoologist 24(3): 116A, 1984

The effects of prostaglandins, phosphodiesterase inhibitors, and cyclic AMP on ovulation of brook trout (Salvelinus fontinalis) oocytes. General and Comparative Endocrinology, 482: 154-160, 1982

Seasonal changes in immune parameters of rainbow trout (Oncorhynchus mykiss), brook trout (Salvelinus fontinalis) and brook trout × Arctic charr hybrids (Salvelinus fontinalis × Salvelinus alpinus alpinus). Fish & Shellfish Immunology 57: 400-405, 2016

Prostaglandin levels during steroid induced in vitro final maturation of brook trout salvelinus fontinalis oocytes. American Zoologist 20(4): 730, 1980

Steroid-induced final maturation in brook trout (Salvelinus fontinalis) oocytes in vitro: the effects of forskolin and phosphodiesterase inhibitors. Biology of Reproduction 36(5): 1321-1332, 1987

The in vitro induction of final maturation and ovulation in brook trout (Salvelinus fontinalis) and yellow perch (Perca flavescens) ova and the hormonal control of final maturation in ova of uther fish species. Dissertation Abstracts lnternational, 3711: 5540-5541, 1977

Patterns of growth and nutrient deposition in lake trout (Salvelinus namaycush), brook trout (Salvelinus fontinalis) and their hybrid, F1 splake (Salvelinus namaycush x Salvelinus fontinalis) as a function of water temperature. Aquaculture Nutrition 13(3): 230-239, 2007

In vitro effects of theophylline and di butyryl adenosine 3 5 cyclic mono phosphoric acid on spontaneous and prostaglandin f 2 alpha induced ovulation of brook trout salvelinus fontinalis oocytes. Biology Of Reproductionppl. 1: 114a, 1980