EurekaMag.com logo
+ Site Statistics
References:
47,893,527
Abstracts:
28,296,643
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Electroporation of mammalian cells with a firefly luciferase expression plasmid: kinetics of transient expression differ markedly among cell types


, : Electroporation of mammalian cells with a firefly luciferase expression plasmid: kinetics of transient expression differ markedly among cell types. Dna 7(8): 557-562

Electroporation in combination with the luciferase reporter system provides a rapid and sensitive means of determining transient expression from plasmid constructs in various mammalian cells. Conditions are described allowing efficient electroporation of several cell types with a commercial electroporation device. Measurements on extracts prepared at various times after electroporation showed a rapid rise in luciferase activity for up to 12 hr, which was followed, in certain cell types, by a rapid decline. With such cells, determinations made after 48-72 hr, as is conventional, would greatly underestimate expression of the luciferase reporter gene. Therefore, to observe optimum activity, the kinetics of expression must be determined for each cell type.


Accession: 005349927

PMID: 3180996

DOI: 10.1089/dna.1.1988.7.557

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

de Wet, J.R.; Wood, K.V.; DeLuca, M.; Helinski, D.R.; Subramani, S., 1987: Firefly luciferase gene: structure and expression in mammalian cells. The nucleotide sequence of the luciferase gene from the firefly Photinus pyralis was determined from the analysis of cDNA and genomic clones. The gene contains six introns, all less than 60 bases in length. The 5' end of the luciferase mRNA w...

de Wet, JR.; Wood, KV.; DeLuca, M.; Helinski, DR.; Subramani, S., 1987: Cloning and expression of the firefly luciferase gene in mammalian cells. Unknown

Ma, X.; Lu, X.; Peng, J.; Tan, S.; Yuan, Z.; Yin, F.; Wang, H.; Wu, X.; Hou, Y., 2003: Inhibition of Luciferase expression in mammalian cells by AAV vector plasmid mediated Luciferase shRNA. Constructing a plasmid containing the shRNA of luciferase to suppress the expression of luciferase in BHK-21 cell. A 334 bp human U6 snRNA promoter was amplified from human genomic DNA by PCR and ligated to a 21 bp reverse repeated motif of lucife...

Ow, D.W.; D.W.t, J.R.; Helinski, D.R.; Howell, S.H.; Wood, K.V.; Deluca, M., 1986: Transient and stable expression of the firefly luciferase gene in plant cells and transgenic plants. The luciferase gene from the firefly, Photinus pyralis, was used as a reporter of gene expression by light production in transfected plant cells and transgenic plants. A complementary DNA clone of the firefly luciferase gene under the control of a...

White, M.R.H.; Morse, J.; Boniszewski, Z.A.M.; Mundy, C.R.; Brady, M.A.W.; Chiswell, D.J., 1990: Imaging of firefly luciferase expression in single mammalian cells using high sensitivity charge coupled device cameras. Quantitative imaging of low-level luminescence and fluorecence is becoming an increasingly important technique for a range of life-science research applications. In particular, charge-coupled device (CCD) cameras are becoming valuable tools for qu...

Hartikka, J.; Cornefert Jensen, F.; Felgner, J.; Kuwahara Rundell, A.Y.; Margalith, M.; Dwarki, V.J.; Vahlsing, L.; Hong, G.; Manthorpe, M., 1994: Studies on firefly luciferase gene expression after intramuscular injection of plasmid DNA. Journal of Cellular Biochemistry Supplement 0(18 PART A): 225

Coker G.T.IIi; Vinnedge L.; O'malley K.L., 1989: 8 bromo cyclic amp inhibits the transient expression of firefly luciferase. Febs Letters 249(2): 183-185

Yee, J.; Nash, T.E., 1995: Transient transfection and expression of firefly luciferase in Giardia lamblia. We have developed a gene transfer system for the protozoan parasite Giardia lamblia. This organism is responsible for many cases of diarrhea worldwide and is considered to be one of the most primitive eukaryotes. Expression of a heterologous gene...

Luehrsen, K.R.; de Wet, J.R.; Walbot, V., 1992: Transient expression analysis in plants using firefly luciferase reporter gene. Methods in Enzymology 216: 397-414

Purdy, J.E.; Mann, B.J.; Pho, L.T.; Petri, W.A., 1994: Transient transfection of the enteric parasite Entamoeba histolytica and expression of firefly luciferase. Development of DNA-mediated transfection in Entamoeba histolytica will facilitate basic research toward the control of this protozoan parasite. A transient transfection system was established by using the firefly luciferase gene ligated to the 5&a...