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Electrostatic and structural properties of the surface of human erythrocytes 2. cell electrophoretical studies following addition of lanthanum chloride

, : Electrostatic and structural properties of the surface of human erythrocytes 2. cell electrophoretical studies following addition of lanthanum chloride. Studia Biophysica 78(2): 143-150

The electrophoretic mobility (EPM) of human erythrocytes as a function of ionic strength was measured following addition of lanthanum chloride. The reduction of the surface charge by adsorption of La ions is essentially affected by the buffer system used. In phosphate buffer systems, adsorption of La ions occurs only at the phosphate groups of the phospholipids. La is adsorbed also within the glycocalyx when using carbonate buffers, leading to stronger reduction of EPM than in phosphate-buffered solutions. With declining pH-values, the reduction of the negative surface charge in phophate buffer solutions increases due to adsorption of La ions. The membrane charge alteration following La3+-treatment was quantitatively determined as (0.7-1.3) .times. 107 positive charges/erythrocyte.

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