Section 6
Chapter 5,353

Elisa for detection of immunoglobulin g and immunoglobulin m antibodies to herpes simplex virus type 1 and herpes simplex virus type 2 in human sera

Katz, D.; Hilliard, J.K.; Mirkovic, R.R.; Word, R.A.

Journal of Virological Methods 14(1): 43-56


ISSN/ISBN: 0166-0934
Accession: 005352249

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A rapid, enzyme-linked immunoassay (ELISA) was applied to identify and measure specific IgG and IgM antibodies to herpes simplex viruses types 1 and 2 (HSV-1 and HSV-2). Detergent solubilized infected cells and mock-infected cells were used as antigens in the assay. Identification of type-specific antibodies was achieved by a competition assay in which clinical sera mixed with HSV-1 or HSV-2 antigens were assayed for reactivity to identical antigens coating wells of polystyrene microtiter plates. Reactivity and the specificity of the reactive immunoglobulin class was quantitated using biotinylated goat anti-IgG and biotinylated goat anti-IgM. Five paired sera from patients with diagnosed herpes simplex genital infections and one human anti-HSV-1 reference serum were tested with this assay and results were compared to results previously obtained using a complement fixation test and micro-SPRIA. The results indicate that the ELISA is a specific, sensitive and simple test which confirms the herpes simplex virus infection history of patients.

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