Endogenous iaa and morphogenesis in tobacco nicotiana tabacum cultivar xanthi nc petiole cultures

Cassells, A.C.; Long, R.D.; Mousdale, D.M.A.

Physiologia Plantarum 56(4): 507-512

1982


ISSN/ISBN: 0031-9317
DOI: 10.1111/j.1399-3054.1982.tb04547.x
Accession: 005358021

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Abstract
Short duration (48 h) dark or high light treatment of the donor plants influenced the pattern of auxin metabolism in tobacco petioles in culture. In explants from dark treated donor plants there was a peak of IAA detectable at day 3 in culture. This was associated with reduced morphogenetic potential of the explant. Inclusion of 2,3,5-triiodobenzoic acid in the medium prevented this IAA accumulation and eliminated the inhibitory effect of the dark pretreatment. Inclusion of IAA in the culture medium reduced the morphogenetic potential of explants from high light treated donor plants but had no effect on the morphogenetic potential of explants from dark treated donor plants. Explants cultured for 1 day on auxin-free medium and then transferred to IAA-containing medium were sensitive to auxin; those transferred after 5 days were insensitive. Studies on the interaction between media sucrose concentrations, endogenous IAA and peroxidase, and morphogenesis are reported. The results are discussed in relation to the influence of endogenous auxin on the receptivity of explants to exogenous (media) morphogenetic stimuli.

Endogenous iaa and morphogenesis in tobacco nicotiana tabacum cultivar xanthi nc petiole cultures