EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Endorphins in several regions of rat brain large differences between radio immunoassay and radio receptor assay



Endorphins in several regions of rat brain large differences between radio immunoassay and radio receptor assay



European Journal of Pharmacology 46(1): 73-74



Various endorphins (i.e., peptide fragments from .beta.-lipotropin) with affinity for the opiate receptors were characterized. Two pentapeptides, methionine-enkephalin (Met-ENK) and leucine-enkephalin (Leu-ENK) were found in brain, and larger fragments, the .alpha.- and .beta.-endorphins (61-76 and 61-91 residues, respectively) in pituitary. Marked regional differences in total endorphins (RRA [radioreceptor assay]) and Met- and Leu-ENK (RIA [radioimmunoassay]) were observed, with similar ranking, for the levels of the 3 groups of compounds when using HCl extraction: highest in striatum and hypothalamus and lowest in hippocampus and cerebellum. Total ENK levels (RIA) in each region represented only 3-20% of the total endorphins (RRA). Because, in contrast with the opiate receptors which have similar affinities for all classes of endorphins, these antisera against ENK did not recognize the larger classes like .alpha.- or .beta.-endorphins; this difference was interpreted as evidence for the presence of significant amounts of the latter compounds in brain regions and not only in pituitary, as previously thought.

(PDF emailed within 1 workday: $29.90)

Accession: 005359062

Download citation: RISBibTeXText

PMID: 923653



Related references

Radio immunoassay of methionine enkephalin and leucine enkephalins in regions of rat brain and comparison with endorphins estimated by a radio receptor assay. Journal of Neurochemistry 31(1): 29-40, 1978

Basic studies and clinical applications of radio receptor assay for hormones and other biologically active substances part 3 determinations of hormonal levels in blood by radio receptor assay and radio immunoassay and the clinical significance of radio receptor assay. Japanese Journal of Medicine 15(3): 291, 1976

Measurement of somatomedin related peptides in fetal neo natal and maternal rat serum by insulin like growth factor i radio immunoassay insulin like growth factor ii radio receptor assay and multiplication stimulating activity radio receptor assay after acid ethanol extraction. Endocrinology 110(2): 575-581, 1982

Specific radio immunoassay and radio receptor assay of human chorionic gonadotropin. Folia Endocrinologica Japonica 50(2): 181, 1974

Comparison of radio receptor assay and radio immunoassay for ovine bovine and porcine fsh. Journal Of Animal Scienceppl. 1: 340, 1982

Radio immunoassay and radio receptor assay for wallaby follicle stimulating hormone. Theriogenology 8(4): 176, 1977

Development of a radio immunoassay for somatomedin c comparison with radio receptor assay. Endocrine Society of Australia Proceedings 24: 56, 1981

Half disappearance time of synthetic somatostatin in man by radio receptor assay and radio immunoassay. Journal of Clinical Chemistry & Clinical Biochemistry 18(10): 734, 1980

Isolation and purification of porcine luteinizing hormone for radio immunoassay and radio receptor assay. Bulletin de l'Academie Polonaise des Sciences Serie des Sciences Biologiques 26(11): 739-744, 1978

Acute somatomedin response to growth hormone radio receptor assay vs. radio immunoassay. Journal of Clinical Endocrinology and Metabolism 52(4): 616-621, 1981

Measurement of rat serum follicle stimulating hormone by radio receptor assay and comparison with radio immunoassay. Endocrinologia Japonica 27(6): 717-726, 1980

Comparison of growth hormone levels in acromegaly determined by radio immunoassay and radio receptor assay. Clinical Research 21(4): 885, 1973

The determination of serum human chorionic gonadotropin by radio immunoassay radio receptor assay and bioassay. Endocrinology 96(SUPPL): 292, 1975

Vasoactive intestinal poly peptide tissue distribution in the rat as measured by radio immunoassay and by radio receptor assay. Annales D'Endocrinologie: 145-146, 1977