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Endosperm protein formation during kernel development of wild type and high lysine barley mutant



Endosperm protein formation during kernel development of wild type and high lysine barley mutant



Cereal Chemistry 53(6): 890-901



The formation of barley [Hordeum vulgare] endosperm proteins was followed by amino acid analysis and SDS[sodium dodecylsulfate]-polyacrylamide gel electrophoresis during kernel development of wild type barley and the high-lysine mutant, Ris.vphi. No. 1508. In the mutant, only minor differences in the amino acid composition and electrophoretic patterns of the albumins and the globulins, respectively, were observed, but 3 times more free amino acids were present at all stages of endosperm development compared to the wild type. Hordein formation was severely impaired and the synthesis of 2 components of the fraction was inhibited in the mutant. The high-lysine content of the mutant endosperm is due to this reduction in the lysine-poor hordeins, a reduction of lysine-poor components of the glutelin fraction, and a compensating increase in the amount of lysine-rich glutelins as well as free lysine.

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Accession: 005359818

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