+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Endosperm protein formation during kernel development of wild type and high lysine barley mutant

Endosperm protein formation during kernel development of wild type and high lysine barley mutant

Cereal Chemistry 53(6): 890-901

The formation of barley [Hordeum vulgare] endosperm proteins was followed by amino acid analysis and SDS[sodium dodecylsulfate]-polyacrylamide gel electrophoresis during kernel development of wild type barley and the high-lysine mutant, Ris.vphi. No. 1508. In the mutant, only minor differences in the amino acid composition and electrophoretic patterns of the albumins and the globulins, respectively, were observed, but 3 times more free amino acids were present at all stages of endosperm development compared to the wild type. Hordein formation was severely impaired and the synthesis of 2 components of the fraction was inhibited in the mutant. The high-lysine content of the mutant endosperm is due to this reduction in the lysine-poor hordeins, a reduction of lysine-poor components of the glutelin fraction, and a compensating increase in the amount of lysine-rich glutelins as well as free lysine.

(PDF emailed within 1 workday: $29.90)

Accession: 005359818

Download citation: RISBibTeXText

Related references

Mrna levels in the developing aleurone and starchy endosperm in wild type and a high lysine lys 3 alpha mutant of barley. Physiologia Plantarum 83(2): 201-208, 1991

Structure and composition of protein bodies from wild-type and high-lysine barley endosperm. Hereditas 81(1): 69-76, 1975

Inheritance of the associated kernel characters high lysine and shrunken endosperm of the barley of cultivar mutant bomi riso 1508. Crop Science 18(5): 828-831, 1978

Effect of latitude on kernel weight and protein and lysine content in normal and shrunken endosperm mutant isotypes of barley. Agronomy abstracts: 80, 1979

In vivo incorporation of lysine into the endosperm proteins of wild type and high-lysine barley. FEBS Letters 52(2): 288-291, 1975

Starch and free sugars during kernel development of Bomi barley and its high-lysine mutant 1508. Seed protein improvement by nuclear techniques Proceedings of two Research Co ordination Meetings, Baden, 28 March 1 April 1977 and Vienna, 26-30 September 1977 Part I Seed protein improvement in cereals: 115-120, 1978

Protein and amino acid metabolism in normal and high-lysine sugary opaque-2 maize endosperm during kernel development. Revista Brasileira de Genetica 5(2): 313-327, 1982

Effects of the sugary-2 endosperm mutant on kernel characteristics, oil, protein, and lysine in Zea mays L. Dissertation Abstracts International, B 37(3): 1063B-1064B, 1976

Protein and amino acid metabolism in normal and high lysine sugary opaque 2 maize zea mays endosperm during kernel development. Revista Brasileira de Genetica 5(2): 313-328, 1982

Distribution of amino acids in bran, embryo and milled endosperm and shifts in storage protein subunits of in vitro-selected and lysine-enhanced mutant and wild type rice. Plant Science (Limerick) 90(2): 145-154, 1993

Variation of kernel protein and lysine in the wild progenitor of barley. Hereditas 96(1): 29-37, 1982

The amino acid composition in endosperm and embryo of a barley variety and its high lysine mutant. Hereditas 87(1): 43-46, 1977

Protein and enzyme levels during development in a high lysine barley mutant. Phytochemistry 19(7): 1323-1328, 1980

Hordein messenger RNA levels in wild type and mutant barley endosperm. Plant Science Letters 29(2/3): 295-304, 1983

In vitro synthesis of barley endosperm proteins on wild type and mutant templates. Carlsberg Research Communications 41(6): 311-320, 1976