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Endothelial cell hybrids and the suppression of factor viii related antigen expression



Endothelial cell hybrids and the suppression of factor viii related antigen expression



British Journal of Haematology 46(4): 613-620



Hybrid somatic cell clones were generated by fusing human vascular endothelial cells in primary culture to cells of 4 rodent lines [mouse connective tissue A9 cells, Chinese hamster lung E36 cells, mouse embryo 3T3-4E cells and Syrian hamster kidney BHK-B1 cells]. Factor VIII related antigen (VIIIR:Ag) was clearly demonstrable in the cultured endothelial cells, even when they had been co-cultured with rodent cells. But in none of 14 hybrid clones was VIIIR:Ag detectable. Isozyme analyses for human chromosome markers show that all the assayed human chromosomes were represented among the hybrids and that various subsets of human chromosomes were deleted from individual hybrid clones. Either VIIIR:Ag production depends on a particular combination of human chromosomes not represented in any of the hybrids or else the rodent cells may contribute some agent which intracellularly blocks VIIIR:Ag expression.

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Accession: 005359907

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