EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Endothelial cell injury in vitro is associated with increased secretion of a molecular weight 43000 glycoprotein ligand



Endothelial cell injury in vitro is associated with increased secretion of a molecular weight 43000 glycoprotein ligand



Journal of Cellular Physiology 127(3): 373-387



A novel, serum albumin-binding glycoprotein of molecular weight (mw) 43,000 (43K protein) was initially purified from the culture medium of bovine aortic endothelial (BAE) cells (Sage, H., Johnson, C., and Bornstein, P., J. Biol. Chem. 259:3993-4007, 1984). Its secretion by normal mesenchymal cells and by transformed cells of both ectodermal and endodermal origin suggested a general role in cellular function. To examine the effect of sublethal injury in vitro on the biosynthesis of 43K protein, BAE cells were exposed to endotoxin. At concentrations which produced minimal cell detachment and lysis, the cells secreted 70-100% more protein compared to control cultures, and the relative increase in 43K protein over total protein was approximately three-fold. A second type of cellular injury, manifested by rapid cellular proliferation and migration in response to sparse plating density (a condition that we have termed 'culture shock'), was also accompanied by a significant increase in the secretion of 43K protein. Pulse-chase studies revealed that the initial product secreted within 1.5 h was of Mr 38,000, and that between 6 and 21 h this molecular was converted to the final form of Mr 43,000. The 43K protein was not associated with RNA of glycosaminoglycan, but appeared to be linked to complex oligosaccharides containing peripheral sialosyl residues. Treatment with tunicamycin produced lower mw forms that displayed reduced affinity for albumin. By immunologic criteria, peptide mapping, and amino acid analysis, the 43K protein was shown to be structurally distinct from several proteins of Mr 40,000-50,000 associated with endothelium or with serum, including tissue factor, a plasminogen anti-activator, and several apolipoproteins. In addition, the 43K protein was not present in the extracellular matrices of endothelial, fibroblastic, or smooth muscle cells, nor was it found in plasma, serum, platelet releasate, or alveolar lavage fluids. These studies identify a unique Mr 43,000 glycoprotein that is associated with cellular stress or injury in vitro. As a secreted but nonmatrix macromolecule, this protein may be part of a 'survival kit' used by the endothelium to cope with cellular injury.

(PDF emailed within 1 workday: $29.90)

Accession: 005359910

Download citation: RISBibTeXText



Related references

Endothelial cell injury in vitro is associated with increased secretion of an Mr 43,000 glycoprotein ligand. Journal of Cellular Physiology 127(3): 373-387, 1986

Monoclonal antibody identification and characterization of a 43000 molecular weight membrane glycoprotein associated with human breast cancer. Cancer Research 46(3): 1306-1317, 1986

Low molecular weight dextran sulfate acts as an endothelial cell-protectant and prevents complement activation as well as ischemia/reperfusion injury in vitro. Molecular Immunology 38(2-3): 105-106, August, 2001

Low molecular weight dextran sulfate prevents complement activation and acts as an endothelial cell-protectant in xenotransplantation and ischemia/reperfusion injury models in vitro. Xenotransplantation 8(Supplement 1): 64, August, 2001

P-selectin glycoprotein ligand-1 inhibition blocks increased leukocyte-endothelial interactions associated with sickle cell disease in mice. Blood 120(18): 3862-3864, 2013

Putative high molecular weight precursor for 43000 molecular weight protein associated with nicotinic acetyl choline receptors. Journal of Cell Biology 97(5 PART 2): 240A, 1983

Endothelial inducible costimulator ligand expression is increased during human cardiac allograft rejection and regulates endothelial cell-dependent allo-activation of CD8+ T cells in vitro. European Journal of Immunology 35(6): 1712-1721, 2005

Alpha thrombin induced tyrosine phosphorylation of 43000 molecular weight and 41000 molecular weight proteins is independent of cytoplasmic alkalinization in quiescent fibroblasts. Biochemical Journal 238(2): 451-458, 1986

Evidence from molecular cloning that sparc a major product of mouse embryo parietal endoderm is related to an endothelial cell culture shock glycoprotein of 4300 molecular weight. EMBO (European Molecular Biology Organization) Journal 5(7): 1465-1472, 1986

Low molecular weight heparin suppresses lymphatic endothelial cell proliferation induced by vascular endothelial growth factor C in vitro. Chinese Medical Journal 122(13): 1570-1574, 2009

Identification of a high molecular weight endothelial cell surface glycoprotein, endoGlyx-1, in normal and tumor blood vessels. Laboratory Investigation; A Journal of Technical Methods and Pathology 71(3): 366-373, 1994

Inhibitory effect of low molecular weight heparin on the secretion of vascular endothelial growth factor by tumor cells in vitro. Zhonghua Zhong Liu Za Zhi 31(11): 826-830, 2010

Interaction between endothelial cells and basement membrane components. In vitro studies on endothelial cell adhesion to collagen types I, III, IV and high molecular weight fragments of IV. Experimental Cell Biology 57(6): 315-323, 1989

Monoclonal antibody to a 43000 molecular weight surface protein of a human leukemia cell line thp 1 cross reacts with the fibroblast intermediate filament protein vimentin. Journal of Cell Science 73: 87-104, 1985

The role of neutrophil membrane glycoprotein 150 (Gp-150) in neutrophil-mediated endothelial cell injury in vitro. Journal of Immunology 135(1): 537-543, 1985