+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Endothelial cell interactions with sickle cell, sickle trait, mechanically injured, and normal erythrocytes under controlled flow

Endothelial cell interactions with sickle cell, sickle trait, mechanically injured, and normal erythrocytes under controlled flow

Blood 70(1): 152-157

Increased adhesive forces between sickle erythrocytes and endothelial cells (EC) have been hypothesized to play a role in the initiation of vasoocclusion in sickle cell anemia. Erythrocyte/human umbilical vein EC interctions were studied under controlled flow conditions for normal (AA), homozygous sickle cell (SS), sickle cell trait (AS), mechanically injured normal, and "high-reticulocyte control" RBC by using video microscopy and digital image processing. The number of adherent RBC was determined at ten-minute intervals during a washout period. Results indicate that SS RBC were more adherent than AA RBC. Mechanically injured (sheared) AA RBC were also more adherent than control normal cells but less adherent than SS RBC. AS RBC did not differ significantly in their adhesive properties from normal RBC. Less-dense RBC were more adherent to EC than dense cells for normal, SS, and high-reticulocyte control RBC. The number of cells adherent at a given time during washout was a very strong function of wall shear rate. In addition, at all shear rates studied, the average velocity of individual SS RBC in the region near the EC surface was approximately half that of AA RBC at the same bulk volumetric flow rate through the flow chamber. These findings suggest that the increased adhesion of sickle RBC is at least partially related to the increased numbers of less-dense RBC present. Increased adherence of the less-dense cells to the EC lining vessel walls could contribute to microvascular occlusions by lengthening vascular transit times of other sickle cells.

(PDF emailed within 1 workday: $29.90)

Accession: 005359911

Download citation: RISBibTeXText

PMID: 3593962

Related references

Individual variability in response to a single sickling event for normal, sickle cell, and sickle trait erythrocytes. Translational Research, 2016

Effect of oxygen tension and of pH on the sickling and mechanical fragility of erythrocytes from patients with sickle cell anemia and the sickle cell trait. Journal of Laboratory and Clinical Medicine 37(5): 789-802, 1951

Plasma lipids in Iranians with sickle cell disease: hypocholesterolemia in sickle cell anemia and increase of HDL-cholesterol in sickle cell trait. Clinica Chimica Acta; International Journal of Clinical Chemistry 365(1-2): 217-220, 2005

Perception of young adults with sickle cell disease or sickle cell trait about participation in the CHOICES randomized controlled trial. Journal of Advanced Nursing 72(6): 1430-1440, 2018

Morphological features of the human umbilical vein in normal, sickle cell trait, and sickle cell disease pregnancies. Human Pathology 30(1): 13-20, 1999

The turnover time of phosphorus in normal, sickle cell trait, and sickle cell anemia blood in vitro as measured with P32 1,2. Science 115(2978): 89-90, 1952

Pneumococcal vaccination coverage among children with sickle cell anemia, sickle cell trait, and normal hemoglobin. Pediatric Blood & Cancer: E27282-E27282, 2018

A study on the glucose uptake, pyruvate and lactate formation in red blood cells of normal, sickle cell trait and sickle cell patients. Indian Journal of Clinical Biochemistry 7(2): 134-137, 1992

Optimal haematocrit in subjects with normal haemoglobin genotype (HbAA), sickle cell trait (HbAS), and homozygous sickle cell disease (HbSS). Clinical Hemorheology and Microcirculation 47(4): 253-260, 2011

Oxygen delivery index in subjects with normal haemoglobin (HbAA), sickle cell trait (HbAS) and homozygous sickle cell disease (HbSS). Clinical Hemorheology and Microcirculation 40(4): 303-309, 2009

Frequency distribution of sickle cell anemia, sickle cell trait and sickle/beta-thalassemia among anemic patients in Saudi Arabia. Journal of Natural Science, Biology, and Medicine 6(Suppl 1): S85-S88, 2015

Novel test method (sickle confirm) to differentiate sickle cell anemia from sickle cell trait for potential use in developing countries. Clinical Laboratory Science 25(1): 26-34, 2012

Examination of the filterability of oxygenated erythrocytes from normal sickle cell trait or sickle cell anemic individuals in the presence of l epinephrine racemic isoproterenol or prostaglandin a 1 prostaglandin a 2 prostaglandin e 1 prostaglandin e 2 prostaglandin f 1 alpha or prostaglandin f 2 alpha. Federation Proceedings 33(5 PART 2): 1396, 1974

Sickle cell trait human erythrocytes are significantly stiffer than normal. Journal of Biomechanics 44(4): 657-661, 2011

The effect of bicarbonate and distilled water on sickle cell trait hematuria and in vitro studies on the interaction of osmolality and pH on erythrocyte sickling in sickle cell trait. Journal of Urology 118(5): 793-796, 1977