+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Endothelial cell monolayer integrity 1. characterization of dense peripheral band of microfilaments

Endothelial cell monolayer integrity 1. characterization of dense peripheral band of microfilaments

Arteriosclerosis 6(2): 212-219

Although the endothelial cell (EC) cytoskeleton has been studied both in vitro and in vivo, little is known about its role in endothelial integrity. We have previously suggested that specific EC microfilament (MF) structure, which we have termed the "dense peripheral band (DPB)," may play a major role in this process. We have extended our studies to characterize this structure in pig aortic ECs in vitro. During the growth of EC cultures, the DPB appears only when the cultures have attained confluency. Using double fluorescent labeling, we found that alpha-actinin, myosin, and tropomyosin colocalized with the F-actin making up the DPB. Occasional microtubules were present in this region, although there was no preferred association between microtubules and the DPB. Colocalization studies revealed vinculin plaques at the cell-cell interface. Thin MFs extended from the DPB into the cytoplasmic side of these plaques. The DPB was completely disrupted by low dose cytochalasin B within 30 minutes, whereas many central MF bundles were still present at 24 hours. The results of this study suggest that the DPB is a distinct structure in the confluent EC monolayer and is closely associated with the ability of ECs to form and maintain the EC monolayer. The disruption of the DPB as an important initial event in the pathogenesis of vascular diseases such as atherosclerosis is discussed.

(PDF emailed within 1 workday: $29.90)

Accession: 005359926

Download citation: RISBibTeXText

PMID: 3954675

Related references

Endothelial monolayer integrity. Perturbation of F-actin filaments and the dense peripheral band-vinculin network. Arteriosclerosis 10(1): 76-84, 1990

The dense peripheral band a microfilament structure associated with endothelial cell monolayer formation. Journal of Steroid Biochemistry 20(6-part-P2): 1, 1984

The role of the dense peripheral band of micro filaments in the maintenance of endothelial integrity. Journal of Cell Biology 97(5 PART 2): 267A, 1983

The dense peripheral band a micro filament bundle associated with endothelial cell confluency. Federation Proceedings 43(4): ABSTRACT 4012, 1984

The dense peripheral band a dynamic microfilament structure present in confluent endothelial cell monolayers. Journal of Cell Biology 99(4 PART 2): 198A, 1984

HPA-1a alloantibodies reduce endothelial cell spreading and monolayer integrity. Molecular Immunology 46(3): 406-415, 2008

The effect of hyaluronate and its oligosaccharides on endothelial cell proliferation and monolayer integrity. Experimental Cell Research 183(1): 179-196, 1989

Chronic ethanol exposure decreases cultured endothelial cell monolayer integrity. FASEB Journal 3(4): A1219, 1989

Vascular endothelial growth factor-C, a potential paracrine regulator of glomerular permeability, increases glomerular endothelial cell monolayer integrity and intracellular calcium. American Journal of Pathology 173(4): 938-948, 2008

Alteration of endothelial cell monolayer integrity triggers resynthesis of vascular endothelium cadherin. Journal of Biological Chemistry 273(45): 29786-29793, 1998

Mercury-arc photolysis: a method for examining second messenger regulation of endothelial cell monolayer integrity. Analytical Biochemistry 196(1): 31-38, 1991

Influence of the fibrin(ogen) degradation product fragment D on the monolayer integrity of cultured vascular endothelial cells. Failure to induce monolayer disorganization. Haemostasis 18(2): 99-105, 1988

Microfilaments and microtubules maintain endothelial integrity. Microscopy Research and Technique 60(1): 115-127, 2002

Dengue virus NS1 protein activates cells via Toll-like receptor 4 and disrupts endothelial cell monolayer integrity. Science Translational Medicine 7(304): 304ra142-304ra142, 2016