EurekaMag.com logo
+ Site Statistics
References:
53,623,987
Abstracts:
29,492,080
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Endothelial cell seeding of prosthetic vascular grafts: early experimental studies with cultured autologous canine endothelium



Endothelial cell seeding of prosthetic vascular grafts: early experimental studies with cultured autologous canine endothelium



Archives of Surgery 115(8): 929-933



Thirteen adult dogs underwent thoracoabdominal bypass operations with 6-mm, double-velour Dacron grafts 25 to 30 cm long. Experimental grafts were seeded with cultured autologous endothelial cells (n = 7). Unseeded grafts served as controls (n = 6). Endothelial cells were harvested from external jugular vein segments using 0.1% trypsin and 0.5% collagenase solutions. Grafts were studied at weeks 2 and 4. Endothelial cell coverage of experimental graft surfaces after two weeks was 60% to 70%, and approximately 80% after four weeks. Immunofluorescence using factor VIII-related antigen confirmed the graft's inner surface to be endothelium. Endothelial cell coverage in control grafts occurred as pannus ingrowth, and never exceeded more than 10% of the conduit surface. Generation of an early endothelial surface in prosthetic grafts is possible in a canine model using cultured autologous cells.

(PDF emailed within 1 workday: $29.90)

Accession: 005359953

Download citation: RISBibTeXText

PMID: 6446893



Related references

Immediate seeding of enzymatically derived endothelium in Dacron vascular grafts. Early experimental studies with autologous canine cells. Archives of Surgery 115(11): 1289-1294, 1980

Cultured autogenous endothelial cell seeding of prosthetic vascular grafts. Surgical Forum 30: 204-206, 1979

Experimental studies on immediate seeding of dacron velour vascular grafts with enzymatically derived autologous endothelium. Journal of Cardiovascular Surgery 21(6): 743-744, 1980

Fibrinolytic profiles after endothelial cell seeding of prosthetic vascular grafts. Fibrinolysis 8(SUPPL 1): 139, 1994

Influence of endothelial cell seeding and antiplatelet drugs on patency of prosthetic vascular grafts. Zilla, P P , R D Fasol And M Deutsch (Ed ) Endothelialization Of Vascular Grafts; 1st European Workshop on Advanced Technologies in Vascular Surgery, Vienna, Austria, November 5-6, 1986 V+257p S Karger Ag: Basel, Switzerland; New York, New York, Usa Illus 57-63, 1987

Fibrinolysis profiles and platelet activation after endothelial cell seeding of prosthetic vascular grafts. Annals of Vascular Surgery 9(6): 542-546, 1995

Isolation and transplantation of autologous circulating endothelial cells into denuded vessels and prosthetic grafts: implications for cell-based vascular therapy. Circulation 108(21): 2710-2715, 2003

Clinical lining of prosthetic femoropopliteal bypass grafts with cultured autologous endothelial cells. Transplantation Proceedings 24(6): 3043-3043, 1992

An improved method of endothelial seeding on small caliber prosthetic vascular grafts coated with natural extracellular matrix. Clinical Materials 13(1-4): 51-55, 1992

An improved method of endothelial seeding on small caliber prosthetic vascular grafts coated with natural extracellular matrix. Clinical Materials 13(1-4): 51-55, 1993

Endothelial cell seeding kinetics under chronic flow in prosthetic grafts. Annals of Vascular Surgery 10(5): 469-475, 1996

Fate of knitted dacron velour vascular grafts seeded with enzymatically derived autologous canine endothelium. Transactions - American Society for Artificial Internal Organs 28: 178-184, 1982

Autologous endothelial cell sodding of microvascular prosthetic grafts. FASEB Journal 7(3-4): A117, 1993

Endothelial cell seeding of polymeric vascular grafts. Frontiers in Bioscience 9: 1412-1421, 2004

Seeding of polytetrafluoroethylene grafts with homologous vs autologous endothelium. 1985