Enhanced proliferation survival and yield of cultured myelin basic protein reactive lewis rat lymph node cells following dual activation with concanavalin a and myelin basic protein
Ovadia, H.; Paterson, P.Y.
Cellular Immunology 65(2): 380-385
Lymph node cells (LNC) from Lewis rats sensitized 7 days previously to myelin basic protein (MBP) exhibit greatly increased survival and undergo much greater proliferation when cultured in the presence of both concanavalin A (Con A) and MBP. Dual activation, in contrast to culture with either the mitogen or the neural antigen, results in a substantially greater yield of lymphoblasts with the capacity to transfer experimental allergic encephalomyelitis (EAE) to normal syngeneic recipients. Dual activation provides a practical advantage over other methods reported to date for securing larger numbers of functional MBP-reactive lymphoid cells for immunologic studies.