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Enzyme activities in thoracic duct lymph and plasma of anesthetized conscious resting and exercising dogs

Enzyme activities in thoracic duct lymph and plasma of anesthetized conscious resting and exercising dogs

European Journal of Applied Physiology & Occupational Physiology 52(2): 188-195

The significance of changes in lymph flow for the extracellular distribution and transport of cellular enzymes and for the level of enzyme activities in plasma was investigated. Specimens of thoracic duct lymph were obtained from an extracorporal lymph shunt in anesthetized, conscious resting and treadmill exercising dogs (6 km .times. h-1 for 1 h). The activity of 10 enzymes and of protein content in lymph and plasma were studied, as well as lymph flow, lymphatic transport and the lymph-plasma ratio of these compounds. Lactate, pH and blood gases were monitored in venous blood. Lymph flow of 0.80 ml .times. min-1 in anesthetized dogs more than doubled (to 1.86 ml .times. min-1) when the animals were conscious and resting. In anesthetized dogs lymph enzyme activity was higher only for enzymes of predominantly hepatic origin, such as choline esterase (CHE) and alanine aminoferase (ALAT) and was lower for aspartate aminotranferase (ASAT) and aldolase (ALD). In conscious dogs, due to activation of the skeletal muscle tissue pump, lymphatic transport of enzymes with rather high activity in skeletal muscle, and of protein, is significantly enhanced. Enzyme activities in plasma did not differ between the groups. Lymph-plasma activity ratios higher than 1 were found for lactate dehydrogenase (LDH), malate dehydrogenase (MDH), ASAT, creatine kinase (CK), ALD and phosphohexose isomerase (PHI). Exercise stimulated lymph flow up to 4.9 ml .times. min-1 and increased the lymphatic activities of those enzymes with a lymph-plasma ratio higher than unity, these enzymes increasing in the plasma due to the highly increased lymphatic transport. Exercise induced changes of enzyme activities in plasma do not necessarily require a pathological enzyme release, but can be explained fully for moderate exercise by the lymphatic influx of enzymes from the interstitial resting enzyme pool in muscle.

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Accession: 005371317

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PMID: 6425059

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