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Enzymological comparison of serum and organ alkaline phosphatases in pig fetuses



Enzymological comparison of serum and organ alkaline phosphatases in pig fetuses



Japanese Journal of Zootechnical Science 53(5): 305-312



The major serum alkaline phosphatase (ALP) isozymes in pig fetuses were investigated with respect to their organs of origin by means of enzymological comparison of ALP in the liver, kidney, small intestine, bone and placenta. The main serum ALP were separated into 3 bands, designated A, B and C, in order of decreasing mobility. Of the 3 bands, bands A and B had similar mobility to small intestinal ALP; band C had the same mobility as small intestinal and hepatic ALP. In the case of neuraminidase treatment, bands A and B of serum ALP showed low sensitivities, but band C had a high value. In tissue ALP, small intestinal ALP were not susceptible to neuraminidase. The fastest moving band of hepatic ALP also showed low sensitivities, but the others were high. In a heat stability test at 56.degree. C, both bands A and B of serum ALP were stable, but band C was unstable. Small intestinal ALP showed high heat stability, but the bone ALP was extremely unstable. With respect to the inhibitory effects of imidazole, serum bands A and B and the small intestinal ALP showed low effects, but serum band C and hepatic, renal, bone and placental ALP showed relatively high effects. With L-phenylalanine, bands A and B of serum ALP and the small intestinal ALP were inhibited to an intermediate degree, and other ALP, including the serum band C and those of the liver, kidney, bone and placenta, were inhibited relatively weakly. With L-homoarginine, bands A and B of serum ALP and small intestinal ALP were resistant to inhibitory effects, but serum band C and hepatic, renal, bone and placental ALP were severely inhibited. Apparently, band A of serum ALP was mainly derived from the small intestinal and partially from the liver, and the serum band B originated from the small intestine. Band C of serum ALP was quite different from bands A and B and was considered to originate from the bone and liver.

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