EurekaMag.com logo
+ Translate

Epidemiological analysis of neisseria gonorrhoeae in west germany by auxotyping and serological classification using monoclonal antibodies


, : Epidemiological analysis of neisseria gonorrhoeae in west germany by auxotyping and serological classification using monoclonal antibodies. Genitourinary Medicine 62(3): 145-150

We evaluated a new serological classification system for Neisseria gonorrhoeae based on monoclonal antibodies directed against epitopes on the outer membrane protein I, in conjunction with auxotyping, to analyse gonococci from two cities in the Federal Republic of Germany. Isolates of N. gonorrhoeae were collected during 1976-8 and 1980-2 in Lubeck, and during 1980-2 in Heidelberg. Between the two study periods in Lubeck, we observed an appreciable decrease in strains of the auxotype that requires arginine, hypoxanthine, and uracil (AHU-) and with serovar class PrIA-1 and the emergence of strains with the proline requiring auxotype and PrIB-I serovar class. Serovar PrIA-1 accounted for 89 (97%) of 92 strains with the AHU- auxotype as opposed to 12 (4%) of 297 strains with other auxotypes (p < 0.0001). Disseminated gonococcal infection was associated with AHU-/PrIA-1 strains. Penicillinase producing N. gonorrhoeae (PPNG) strains belonged to eight different auxotype and serovar classes, which indicated that different strains had been imported. Classification of strains of N. gonorrhoeae by auxotype and serovar class permits analysis of temporal changes in gonococcal populations, and of migrations of gonococci between different geographical areas. Typing N. gonorrhoeae, together with assessing susceptibilities, may prove useful for further studies of the epidemiology and control of gonorrhoea.

(PDF 0-2 workdays service)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 005375006

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Kohl, P.K.; Knapp, J.S.; Hofmann, H.; Gruender, K.; Petzoldt, D.; Tams, M.R.; Holmes, K.K., 1986: Epidemiological analysis of Neisseria gonorrhoeae in the Federal Republic of Germany by auxotyping and serological classification using monoclonal antibodies. We evaluated a new serological classification system for Neisseria gonorrhoeae based on monoclonal antibodies directed against epitopes on the outer membrane protein I, in conjunction with auxotyping, to analyse gonococci from two cities in the Fe...

Bäckman, M.; Rudén, A.K.; Bygdeman, S.M.; Falk, E.S.; Jonsson, A.; Kallings, I.; Ringertz, O.; Sandström, E.G., 1987: Comparison between serological classification and auxotyping in the analysis of Neisseria gonorrhoeae infections. Auxotyping and serological classification, using monoclonal antibodies, were performed on 730 gonococcal strains. These strains were isolated from 725 consecutive patients seen at the Venereal Outpatients Clinic at the Department of Dermatology, S...

Tam, M.R.; Buchanan, T.M.; Sandström, E.G.; Holmes, K.K.; Knapp, J.S.; Siadak, A.W.; Nowinski, R.C., 1982: Serological classification of Neisseria gonorrhoeae with monoclonal antibodies. Hybrid cells producing monoclonal antibodies against antigens of Neisseria gonorrhoeae were obtained by the polyethylene glycol-mediated fusion of mouse myeloma cells and lymphocytes from mice immunized with gonococcal protein I or outer membrane...

Knapp, J.S.; Tam, M.R.; Nowinski, R.C.; Holmes, K.K.; Sandström, E.G., 1984: Serological classification of Neisseria gonorrhoeae with use of monoclonal antibodies to gonococcal outer membrane protein I. Identification of strain-specific markers on Neisseria gonorrhoeae that are capable of differentiating gonococci into a large number of distinct classes could facilitate analysis of patterns of gonorrhea transmission and application of gonorrhea c...

Agarwal, S.K.; Deb, M.; Prakash, K.; Sharma, A.K., 1992: Auxotyping of Neisseria gonorrhoeae as an additional epidemiological marker. Auxotypes and penicillin sensitivity of 102 strains of N. gonorrhoeae were studied. Ten distinct auxotypes on the basis of growth requirement to seven amino acids were observed. The commonest pattern seen was zero auxotype (33.3%), followed by aux...

Sandström, E.; Bygdeman, S., 1987: Serological classification of Neisseria gonorrhoeae. Clinical and epidemiological applications. The development of gonococcal serovar determination and the different nomenclatures are presented. The current prospects for the application of typing to clinical, epidemiological and theoretical studies are reviewed. Future prospects are discusse...

Coghill, D.V.; Young, H., 1987: Serological classification of Neisseria gonorrhoeae with monoclonal antibody coagglutination reagents. A total of 357 clinical isolates of Neisseria gonorrhoeae from 286 patients were classified serologically using two independently developed panels of monoclonal coagglutination reagents. The Pharmacia (Ph) Diagnostics panel comprised 14 reagents,...

Chu, M.L.; Lin, M.H.; Ho, L.J., 1989: Epidemiological characterization of Neisseria gonorrhoeae isolates from Taiwan. Part II: Distribution of auxotyping, serogrouping and plasmid profile. Epidemiology of gonococcal infection in Taiwan was investigated. Six hundred twelve isolates from 7 cities in 1983-1984 were examined for auxotyping by Hendry and Stewart's method, for serotyping by coagglutination of monoclonal antibodies wi...

Bygdeman, S.; Kallings, I.; Danielsson, D., 1981: Serogrouping and auxotyping for epidemiological study of beta-lactamase-producing Neisseria gonorrhoeae strains isolated in Sweden. Acta Dermato-Venereologica 61(4): 329-334

Knapp J.S.; Sandstrom E.G.; Perine P.L.; Lind I.; Piot P.; Miyamoto Y.; Cossins Y.; D.L.C.uz E.; Viarengo J.; E.A., 1983: Serological classification and epidemiology of neisseria gonorrhoeae using mono clonal antibodies. Abstracts of the Annual Meeting of the American Society for Microbiology 83: C28