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Epidemiological differentiation of Serratia marcescens: typing by bacteriocin production






Applied Microbiology 23(2): 218-225

Epidemiological differentiation of Serratia marcescens: typing by bacteriocin production

A new method for comparing and differentiating strains of S. marcescens is described which has proved useful in determining the epidemiology of hospital infections. Strains were grown in Trypticase soy broth, and bacteriocin production was induced with mitomycin C for 5 hr. The bacteriocin lysates were then spotted onto nine standard indicator strains, which were chosen with the aid of computer analysis from the 118 indicators tested. After 24 hr at 37 C, zones of inhibition due to bacteriocins were recorded. One hundred twentynine strains were differentiated into 72 different bacteriocin production patterns, but 11 strains were nontypable. None of the 45 other strains of Enterobacteriaceae produced bacteriocins. Bacteriocin production was a stable epidemiological marker. Colonial mutants always had identical patterns, as did the same strain which has passed from patient to patient through cross-infection. The new technique does not require any specialized equipment and can be used in laboratories with limited budgets. The applications of the new method in cross-infection studies and as a supplement to serological typing are discussed.


Accession: 005375227

PMID: 4552884



Related references

Farmer, J.J., 1972: Epidemiological differentiation of Serratia marcescens: typing by bacteriocin sensitivity. Strains of Serratia marcescens were compared and differentiated by a new method. Bacteriocin lysates were prepared from mitomycin-induced S. marcescens and added to lawns of test strains. From 100 bacteriocin producers, 12 were chosen with the aid...

Nasu, M., 1978: Bacteriocin (marcescin) typing of Serratia marcescens. II. The distribution of Serratia marcescens in hospital and its isolated cases by bacteriocin (marcescin) typing. Kansenshogaku Zasshi. Journal of the Japanese Association for Infectious Diseases 52(6): 183-189

Traub, W.H.; Raymond, E.A., 1971: Epidemiological surveillance of Serratia marcescens infections by bacteriocin typing. During 10 months, 155 isolates of Serratia marcescens were cultured from 105 patients, of whom 49 were considered to have significant infection. The 155 isolates were typed by bacteriocin sensitivity, and 137 (88.4%) were assigned to 37 provisiona...

Nasu, M.; Goto, J.; Goto, I., 1982: An epidemiological study of Serratia marcescens infections by bacteriocin typing. Bacteriocin sensitivity typing according to the method of Traub (Appl. Microbiol. 1971. 21: 837-840) was carried out on 226 clinical isolates of Serratia marcescens obtained from inpatients at Nagasaki University Hospital during the period from Ja...

Nasu, M., 1981: Epidemiological studies of Serratia marcescens infections by bacteriocin (marcescin) typing. S. marcescens strains isolated from various clinical materials and the environment in university hospital from April 1970 to May 1979 were classified by bacteriocin (marcescin) production typing by the agar cross streakin method utilizing 8 indica...

Traub, W.H.; Kleber, I., 1974: Continued epidemiological surveillance of Serratia marcescens infections by bacteriocin typing, with particular reference to strains isolated at Erlangen. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Erste Abteilung Originale. Reihe A: Medizinische Mikrobiologie und Parasitologie 229(3): 372-382

Nasu, M., 1977: Study on bacteriocin (marcescin) typing of Serratia marcescens. I. Fundamental studies on bacteriocin (marcescin) production and sensitivity. Kansenshogaku Zasshi. Journal of the Japanese Association for Infectious Diseases 51(9): 487-498

Kleber I., 1974: Continued epidemiological surveillance of serratia marcescens infections by bacteriocin typing with particular reference to strains isolated at erlangen west germany. Zentralblatt Fuer Bakteriologie Parasitenkunde Infektionskrankheiten Und Hygiene Erste Abteilung Originale Reihe A Medizinische Mikrobiologie Und Parasitologie: 372-382

Nasu, M.; Goto, J.; Goto, Y.; Tashiro, T.; Itoga, T.; Yamaguchi, K., 1984: Distribution of clinical isolates of Serratia marcescens by bacteriocin typing: a comparison of bacteriocin types between two distant university hospitals. Kansenshogaku Zasshi. Journal of the Japanese Association for Infectious Diseases 58(4): 312-317

Lai, P.S.; Ngeow, Y.F.; Puthucheary, S.D.; Wang, C.W., 1983: Comparison of two methods for bacteriocin typing of Serratia marcescens. Two methods of bacteriocin susceptibility typing for Serratia marcescens were compared. A total of 80 epidemiologically unrelated isolates from patients in a single hospital were typed by the cross-streaking method and the mitomycin C-induced (spo...