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Epidemiological differentiation of Serratia marcescens: typing by bacteriocin sensitivity


, : Epidemiological differentiation of Serratia marcescens: typing by bacteriocin sensitivity. Applied Microbiology 23(2): 226-231

Strains of Serratia marcescens were compared and differentiated by a new method. Bacteriocin lysates were prepared from mitomycin-induced S. marcescens and added to lawns of test strains. From 100 bacteriocin producers, 12 were chosen with the aid of computer analysis as the most useful in differentiation. Uniform drops of the 12 standard bacteriocins were added simultaneously with a bacteriocin-bacteriophage dropper to each strain to be typed. All 93 strains of S. marcescens tested were typable and were differentiated into 79 different sensitivity patterns. One pattern had three strains, 12 patterns had two strains each, and 66 patterns had only one strain. The bacteriocins also inhibited Shigella, Klebsiella, and Enterobacter, but no other Enterobacteriaceae. Bacteriocin sensitivity was less stable as an epidemiological marker than bacteriocin production. Several colonial mutants had sensitivity patterns different from the wild types, but most mutants were identical. In three different instances when cross-infection had been shown by other methods, bacteriocin sensitivity also gave the correct epidemiological results. Until the significance and frequency of genetic variations are known, a more stable epidemiological technique should be used in conjunction with bacteriocin sensitivity.

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Accession: 005375228

PMID: 4552885

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Related references

Farmer, J.J., 1972: Epidemiological differentiation of Serratia marcescens: typing by bacteriocin production. A new method for comparing and differentiating strains of S. marcescens is described which has proved useful in determining the epidemiology of hospital infections. Strains were grown in Trypticase soy broth, and bacteriocin production was induced...

Nasu, M., 1978: Bacteriocin (marcescin) typing of Serratia marcescens. II. The distribution of Serratia marcescens in hospital and its isolated cases by bacteriocin (marcescin) typing. Kansenshogaku Zasshi. Journal of the Japanese Association for Infectious Diseases 52(6): 183-189

Nasu, M.; Goto, J.; Goto, I., 1982: An epidemiological study of Serratia marcescens infections by bacteriocin typing. Bacteriocin sensitivity typing according to the method of Traub (Appl. Microbiol. 1971. 21: 837-840) was carried out on 226 clinical isolates of Serratia marcescens obtained from inpatients at Nagasaki University Hospital during the period from Ja...

Traub, W.H.; Raymond, E.A., 1971: Epidemiological surveillance of Serratia marcescens infections by bacteriocin typing. During 10 months, 155 isolates of Serratia marcescens were cultured from 105 patients, of whom 49 were considered to have significant infection. The 155 isolates were typed by bacteriocin sensitivity, and 137 (88.4%) were assigned to 37 provisiona...

Nasu, M., 1981: Epidemiological studies of Serratia marcescens infections by bacteriocin (marcescin) typing. S. marcescens strains isolated from various clinical materials and the environment in university hospital from April 1970 to May 1979 were classified by bacteriocin (marcescin) production typing by the agar cross streakin method utilizing 8 indica...

Nasu, M., 1977: Study on bacteriocin (marcescin) typing of Serratia marcescens. I. Fundamental studies on bacteriocin (marcescin) production and sensitivity. Kansenshogaku Zasshi. Journal of the Japanese Association for Infectious Diseases 51(9): 487-498

Traub, W.H.; Kleber, I., 1974: Continued epidemiological surveillance of Serratia marcescens infections by bacteriocin typing, with particular reference to strains isolated at Erlangen. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Erste Abteilung Originale. Reihe A: Medizinische Mikrobiologie und Parasitologie 229(3): 372-382

Kleber I., 1974: Continued epidemiological surveillance of serratia marcescens infections by bacteriocin typing with particular reference to strains isolated at erlangen west germany. Zentralblatt Fuer Bakteriologie Parasitenkunde Infektionskrankheiten Und Hygiene Erste Abteilung Originale Reihe A Medizinische Mikrobiologie Und Parasitologie: 372-382

Nasu, M.; Goto, J.; Goto, Y.; Tashiro, T.; Itoga, T.; Yamaguchi, K., 1984: Distribution of clinical isolates of Serratia marcescens by bacteriocin typing: a comparison of bacteriocin types between two distant university hospitals. Kansenshogaku Zasshi. Journal of the Japanese Association for Infectious Diseases 58(4): 312-317

Lai, P.S.; Ngeow, Y.F.; Puthucheary, S.D.; Wang, C.W., 1983: Comparison of two methods for bacteriocin typing of Serratia marcescens. Two methods of bacteriocin susceptibility typing for Serratia marcescens were compared. A total of 80 epidemiologically unrelated isolates from patients in a single hospital were typed by the cross-streaking method and the mitomycin C-induced (spo...