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Equine influenza antigenic comparison of inactivated vaccines using acetyl ethylenimine beta propio lactone and formalin

Equine influenza antigenic comparison of inactivated vaccines using acetyl ethylenimine beta propio lactone and formalin

Arquivos da Escola de Veterinaria Universidade Federal de Minas Gerais 34(2): 313-324

Influenza A/equi 1/RJ (Heq1Neq1) virus-infected allantoic fluid from embryonated eggs was concentrated by absorption to and elution from chicken red cells. Three different concentrates were used for preparation of 3 vaccines, using acetylethyleneimine (AEI), .beta.-propiolactone (BPL) and formalin for virus inactivation. Hemagglutination (HA) and infectivity titrations were performed before inactivation. The antigenicity of the 3 vaccines was estimated by inoculation into roosters and later determination of hemagglutination-inhibition (HAI) antibodies in their sera. Each vaccine was inoculated undiluted and diluted (1:3.33 and 1:10) using 3 roosters for each dilution. Statistical analysis of rooster HAI titers indicated that the AEI-inactivated vaccine was the least efficient; only 1 of the 3 roosters inoculated with a 1:10 dilution developed antibodies. Comparing undiluted and 1:10 diluted BPL- and formalin-inactivated vaccines, the BPL vaccine induced higher titers. Formalin-inactivated vaccine was slightly superior when diluted 1:3.33. HAI titers, in general, the BPL-inactivated vaccine displayed the best average, followed by the formalin- and AEI-inactivated ones. No statistically significant difference was found between the results from the 2 bleedings. Among the 3 vaccines, BPL showed the lowest HA and infectivity titers, although it provoked antibodies at higher levels. This could be explained by a minor protein alteration caused by BPL and, therefore, a better antigenicity.

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