Establishment and characterization of long term embryogenic maize zea mays callus and cell suspension cultures

Kamo, K.K.; Hodges, T.K.

Plant Science 45(2): 111-118


ISSN/ISBN: 0168-9452
Accession: 005384566

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Friable callus (type 2) was selected from three genotypes (A188, hybrid .times. A188 .times. B73, and hybrid B73 A188) of Zea mays L. The three genotypes of type 2 callus doubled in fresh weight after 1 week, and growth was better on N6 than on Murashige-Skoog (MS) medium. Type 2 callus of hybrid B73 .times. A188 was maintained in culture longer than A188 type 2 callus, and it regenerated higher numbers of plants than the other two genotypes. Type 2 callus of the hybrid B73 .times. A188 was used to establish cell suspensions. Suspension cells initially grew better on N6 than on MS medium, but after several months of subculture, cells in either N6 or MS medium grew at similar rates. Suspension cells were in mid-log phase by 5-7 days and in stationary phase by about 10 days depending on inoculum density. Growth rate was optimal when cells were transferred at mid-low phase and dry weight of the suspension cells increased at least 10-fold during a 10-day period. Suspension cells from 9-month-old cultures plated on solid medium regenerated plants at an efficiency similar to that of the friable type 2 callus but with more phenotypic abnormalities. Thus, cell suspensions derived from type 2 B73 .times. A188 callus, in culture for over 1 year, were capable of regenerating plants when 9-months old.