Esterase 16 ec es 16 characterization polymorphism and linkage to chromosome 3 of a kidney esterase locus of the house mouse

Van-Deimling, O.H.; Schupp, P.; Otto, J.

Biochemical Genetics 19(11-12): 1091-1100


ISSN/ISBN: 0006-2928
Accession: 005385412

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A polymorphism for an isozyme of a presumed arylesterase, esterase-16 (EC, was detected in kidney, heart and spleen of the house mouse, Mus musculus, by means of isoelectric focusing and by disc electrophoresis. Three phenotypes can be distinguished: the ES-16A phenotype (IEP 5.9) was found in C57BL/10Sn and many other laboratory inbred strains; the ES-16B phenotype (IEP 6.1) was found in M. m. molossinus; and the ES-16C phenotype (IEP 5.9; very weak activity) was found in Peru-Coppock. Esterase-16 is strongly inhibited by 10-3 M p-chloromercuribenzoate, but not by 2 .cntdot. 10-4 M bis-p-nitrophenyl phosphate or by 10-3 M Diamox. It stains well with indoxyl acetate and other indigogenic substrates but only weakly with .alpha.-naphthyl acetate. Esterase-16 is completely insoluble in water. It is apparently governed by a structural gene locus, Es-16, with 3 alleles, Es-16a, Es-16b and Es-16c, respectively. Es-16 is closely linked to Car-1 and Car-2 on chromosome 3. Typing of 94 animals of the backcross (C57BL/10Sn .times. M. m. molossinus) F1 .times. M. m. molossinus revealed a recombination frequency of 8.51 .+-. 2.9%.