Estimation of aneuploidy levels in human spermatozoa using chromosome specific probes and in situ hybridisation

Joseph, A.M.; Gosden, J.R.; Chandley, A.C.

Human Genetics 66(2-3): 234-238

1984


ISSN/ISBN: 0340-6717
PMID: 6201432
DOI: 10.1007/bf00286608
Accession: 005386920

Download citation:  
Text
  |  
BibTeX
  |  
RIS

Article/Abstract emailed within 0-6 h
Payments are secure & encrypted
Powered by Stripe
Powered by PayPal

Abstract
An attempt is described to estimate the frequency of aneuploid human spermatozoa with disomic Y chromosome and disomic chromosome 1 complements, using chromosome specific probes and in situ hybridization. This approach was used as an alternative to the differential staining techniques that were applied to spermatozoa in previous studies aimed at estimating levels of aneuploidy for chromosome 1 and the Y chromosome. A frequency of 1.8/1000 YY-bearing spermatozoa and 3.5/1000 disomy 1 spermatozoa was found, both figures being in excess of those found by sperm genome karyotyping. The technical limitations of the method are discussed.

Estimation of aneuploidy levels in human spermatozoa using chromosome specific probes and in situ hybridisation