EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Estrogen induced destabilization of yolk precursor protein messenger rna in avian liver


Journal of Biological Chemistry 263(6): 2625-2631
Estrogen induced destabilization of yolk precursor protein messenger rna in avian liver
In addition to regulating gene expression at the level of transcription, estrogen is generally considered to selectively stabilize induced mRNAs against degradation. As a result of mRNA stabilization, estrogen-induced mRNAs accumulate to much higher levels in target cells, and the encoded proteins are made at much greater rates than would occur on the basis of transcriptional activation alone. The present study examined the effect of estrogen on the stabilities of avian liver mRNAs that code for the yolk precursor proteins apolipoprotein (apo) II and vitellogenin (VTG) II. The results show that the degradation rates of apoII and VTGII mRNAs during hormone withdrawal are dramatically altered by the duration of prior estrogen treatment. During the 2 days required for the hormonal inductions of these mRNAs to new steady states, the turnover rates of both mRNAs were the same in the presence and absence of estrogen (t1/2 = 13 h). This result indicates that mRNA stabilization does not contribute to the extensive accumulation of apoII and VTG II mRNAs. When the duration of estrogen treatment was extended beyond 3 days, however, hormone withdrawal led to the rapid (t1/2 = 1.5 h) and selective destabilization of these mRNAs. This result suggests that estrogen induced a destabilization activity that was only functional followihg hormone withdrawal. Thus, the point at which estrogen alters mRNA stabiliity is at the level of mRNA degradation. An absence of detectable apoII mRNA degradation intermediates during either the slow or rapid mode of mRNA decay suggests that the rate-limiting step for apoII mRNA turnover is an estrogen-sensitive targeting event that marks the mRNA for rapid degradation.


Accession: 005389847



Related references

Estrogen-induced destabilization of yolk precursor protein mRNAs in avian liver. Journal of Biological Chemistry 263(6): 2625-2631, 1988

Estrogen regulation of yolk and non-yolk protein synthesis in the avian liver. An immunocytochemical study. Differentiation; Research in Biological Diversity 18(2): 105-114, 1981

Developmental regulation of the estrogen receptor and the estrogen responsiveness of five yolk protein genes in the avian liver. Proceedings of the National Academy of Sciences of the United States of America 84(23): 8493-8497, 1987

Kinetics of primary and secondary stimulation of the messenger rna for very low density lipo protein apo lipo protein ii a major yolk protein in the cockerel liver by estrogen. Endocrinology 113(3): 1158-1160, 1983

Estrogen induction of very low density apolipoprotein II synthesis, a major avian liver yolk protein, involves the recruitment of hepatocytes. Endocrinology 118(2): 538-544, 1986

Decrease in functional albumin messenger rna during estrogen induced vitellogenin biosynthesis in avian liver. Proceedings of the National Academy of Sciences of the United States of America 75(12): 5974-5978, 1978

Detection and characterization of degradative intermediates of avian apo very low density lipoprotein ii messenger rna present in estrogen treated birds and following destabilization by hormone withdrawal. Journal of Biological Chemistry 264(11): 6495-6503, 1989

Estrogen induced destabilization and associated degradation intermediates of apolipoprotein ii messenger rna. Sato, G H And J L Stevens (Ed ) Progress in Clinical And Biological Research, Vol 322 Molecular Endocrinology And Steroid Hormone Action; Fourth International Symposium on Cellular Endocrinology, Lake Placid, New York, Usa, August 24-27, 1988 Xix+320p Alan R Liss, Inc : New York, New York, Usa Illus 227-240, 1989

Translation of hormone-induced messenger RNA in amphibian oocytes: I. Induction by estrogen of messenger RNA encoded for vitellogenic protein in the liver of the male African clawed toad (Xenopus laevis). Proceedings of the National Academy of Sciences of the United States of America 72(10): 3934-3938, 1975

Translation of hormone induced messenger rna in amphibian oocytes part 1 induction by estrogen of messenger rna encoded for vitellogenic protein in the liver of the male african clawed toad xenopus laevis. Proceedings of the National Academy of Sciences of the United States of America 72(10): 3934-3938, 1975