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Evaluation of a competitive binding assay for cortisol using horse transcortin






Endokrinologie 72(2): 214-222

Evaluation of a competitive binding assay for cortisol using horse transcortin

A non-chromatographic competitive binding assay (CBA) using horse transcortin has been employed in the routine measurement of cortisol in plasma, urine and amniotic fluids. Comparing the values with those of a radioimmunoassay (RIA) or a fluorimetric method (FM) an excellent correlation between the three methods both in plasma and urine has been calculated in normal subjects and in patients with various endocrine disorders. In amniotic fluids, however, there were discrepancies between CBA and RIA. Whereas CBA showed no differences, RIA gave significantly higher values in amniotic fluids of female than of male fetuses. Elevated free plasma cortisol levels observed in patients with prostatic cancer after diethyl stilboestrol diphosphate therapy did not correlate with unconjugated urinary cortisol concentration as measured with CBA and FM. In newborns, a relatively high plasma level found 12 hours after birth was followed by a nadir on the 2nd and 3rd day of life and by an increase until levels of adults on the 5th day of life were reached.


Accession: 005395004

PMID: 570104



Related references

Dash R.J.; Sharma B.R.; Lata V., 1979: Radio ligand binding assay of cortisol using horse transcortin. A modified radioligand binding assay was developed to measure cortisol [C] in a single methylene chloride extract of human plasma/serum. The assay utilizes 5% horse serum for C binding and 3H-C as tracer. Except for a cross reaction of 13.3% for c...

Pham-Huu-Trung, M.T., 1970: Determination of plasma cortisol by competitive binding with transcortin. Annales de Biologie Clinique 28(2): 145-152

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Fang V.S.; Warenica B., 1982: Kit radio immunoassay for determination of plasma cortisol and urinary free cortisol compared with competitive protein binding assay. Clinical Chemistry 28(7): 1653