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Evaluation of a competitive binding assay for cortisol using horse transcortin


, : Evaluation of a competitive binding assay for cortisol using horse transcortin. Endokrinologie 72(2): 214-222

A non-chromatographic competitive binding assay (CBA) using horse transcortin has been employed in the routine measurement of cortisol in plasma, urine and amniotic fluids. Comparing the values with those of a radioimmunoassay (RIA) or a fluorimetric method (FM) an excellent correlation between the three methods both in plasma and urine has been calculated in normal subjects and in patients with various endocrine disorders. In amniotic fluids, however, there were discrepancies between CBA and RIA. Whereas CBA showed no differences, RIA gave significantly higher values in amniotic fluids of female than of male fetuses. Elevated free plasma cortisol levels observed in patients with prostatic cancer after diethyl stilboestrol diphosphate therapy did not correlate with unconjugated urinary cortisol concentration as measured with CBA and FM. In newborns, a relatively high plasma level found 12 hours after birth was followed by a nadir on the 2nd and 3rd day of life and by an increase until levels of adults on the 5th day of life were reached.

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Accession: 005395004

PMID: 570104

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Related references

Dash R.J.; Sharma B.R.; Lata V., 1979: Radio ligand binding assay of cortisol using horse transcortin. A modified radioligand binding assay was developed to measure cortisol [C] in a single methylene chloride extract of human plasma/serum. The assay utilizes 5% horse serum for C binding and 3H-C as tracer. Except for a cross reaction of 13.3% for c...

Pham-Huu-Trung, M.T., 1970: Determination of plasma cortisol by competitive binding with transcortin. Annales de Biologie Clinique 28(2): 145-152

Pegg, P.J.; Keane, P.M., 1969: The simultaneous estimation of plasma cortisol and transcortin binding characteristics by a competitive protein binding technique. A slight modification of the competitive protein binding assay of Murphy (13) allows the simultaneous determination of plasma cortisol, transcortin binding capacity (Pt) and apparent transcortin-cortisol association constant (Kt). Pt and Kt may he...

D.M.or, P.; Heyns, W., 1968: Cortisol binding affinity of plasma transcortin (CBA) as studied by competitive adsorption. Journal of Clinical Endocrinology and Metabolism 28(9): 1281-1286

D.M.or P.; Heyns W., 1968: Cortisol binding affinity of plasma transcortin as studied by competitive adsorption human estrogen metab. Journal of Clinical Endocrinology & Metabolism 28(9): 1281-1294

Olgaard, K.; Madsen, S., 1976: Evaluation of a simple cortisol assay by competitive protein binding technique. The evaluation of a recently introduced kit (Cortipac) for measuring plasma cortisol by competitive protein binding technique without prior extraction of steroids from plasma, is presented. The blank value was < 2.5 .mu.g/100 ml and the lowest...

Golikov P.P., 1982: Direct assay of hydrocortisone in the blood plasma by the method of competitive binding with endogenous transcortin. A method for direct assay of hydrocortisone in individual blood samples from urgent surgical and therapeutic patients was developed. The method is based on competitive protein binding. Endogenous transcortin of each sample of blood plasma assayed...

Rosenthal, H.E.; Albert, M.; Ludwig, G.; Strumlauf, E.; Sandberg, A.A., 1982: Transcortin: a corticosteroid-binding protein of plasma-XIV. Effects of time of equilibration of serum with cortisol on transcortin determinations by gel filtration. The results of determination of plasma transcortin levels by gel filtration are subject to assay conditions such as duration of incubation and the amount of cortisol added. We investigated the cause for the dependency of results on assay condition...

Rosenthal, H.E.; Albert, M.; Ludwig, G.; Strumlauf, E.; Sandberg, A.A., 1982: Transcortin a cortico steroid binding protein of plasma 14. effects of time of equilibration of serum with cortisol on transcortin determinations by gel filtration. The results of determinations of plasma transcortin levels by gel filtration are subject to assay conditions such as duration of incubation and the amount of cortisol added. The cause for the dependency of results on assay conditions was investiga...

Fang V.S.; Warenica B., 1982: Kit radio immunoassay for determination of plasma cortisol and urinary free cortisol compared with competitive protein binding assay. Clinical Chemistry 28(7): 1653