EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Evaluation of a competitive elisa for porcine escherichia coli heat stable enterotoxin


Journal of Clinical Microbiology 22(6): 893-896
Evaluation of a competitive elisa for porcine escherichia coli heat stable enterotoxin
A competitive enzyme-linked immunosorbent assay (ELISA) was compared with the conventional suckling mouse assay for the identification of heat-stable enterotoxin (STa) in samples from piglets suffering from diarrhea. A total of 110 Escherichia coli isolates, 22 primary cultures, and 26 fecal samples from piglets up to 8 weeks of age with diarrhea were compared in parallel by both assays. Of the 110 isolates tested, all gave consistent results by the ELISA and the suckling mouse assay; 60 strains were negative and 50 strains produced STa by both tests. Identical results were obtained when 22 primary agar cultures were screened for STa production by both methods; 6 were found to produce STa, while 16 did not. When 26 fecal samples were tested for the presence of STa, 10 were negative and 12 were positive by both assays. One of the remaining four samples gave questionable positive results by both the suckling mouse assay and the ELISA, but E. coli. isolated from this samples gave positive results by both tests. The remaining three samples were negative by the suckling mouse assay, but gave questionable positive results by the ELISA. E. coli isolates from these three samples were always negative by both assays. The ELISA used in this study provides a reliable and convenient method for diagnosing STa-producing enterotoxigenic E. coli of porcine origin.


Accession: 005395005



Related references

Evaluation of a competitive elisa for detection of escherichia coli heat stable enterotoxin. Abstracts of the Annual Meeting of the American Society for Microbiology 86: 374, 1986

Evaluation of a competitive enzyme-linked immunosorbent assay for porcine Escherichia coli heat-stable enterotoxin. Journal of Clinical Microbiology 22(6): 893-896, 1985

Development of a competitive enzyme-linked immunosorbent assay (ELISA) for Escherichia coli heat-stable enterotoxin (STa). Journal of Immunological Methods 75(2): 295-307, 1984

Monoclonal antibodies against escherichia coli heat stable toxin and their use in a diagnostic heat stable enterotoxin ganglioside gm 1 elisa. Journal of Clinical Microbiology 24(4): 585-590, 1986

Immunization of swine with heat-stable Escherichia coli enterotoxin coupled to a carrier protein does not protect suckling pigs against an Escherichia coli strain that produces heat-stable enterotoxin. Infection and Immunity 39(2): 990-992, 1983

Elisa for escherichia coli heat stable enterotoxin type ii. Journal of Clinical Microbiology 26(8): 1555-1560, 1988

Detection of the porcine Escherichia coli heat-labile enterotoxin (LT) by ELISA. Buletinul Universitatii de Stiinte Agricole si Medicina Veterinara Cluj Napoca, Seria Medicina Veterinara 60: 58-63, 2003

Detection of Escherichia coli producing heat-stable enterotoxin II by using ELISA and PCR. Japanese Journal of Toxicology & Environmental Health 41(1): 67-76, 1995

Gm 1 elisa method for demonstration of escherichia coli heat stable enterotoxin. FEMS Microbiology Letters 30(1-2): 1-6, 1985

ELISA for detection of heat stable enterotoxin producing Escherichia coli strains. Indian Journal of Medical Research 93: 286-288, 1991