+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Evaluation of a direct fluorescent antibody staining method for rapid identification of members of the bacteroides fragilis group

, : Evaluation of a direct fluorescent antibody staining method for rapid identification of members of the bacteroides fragilis group. American Journal of Clinical Pathology 76(1): 78-82

A direct fluorescent antibody test kit (Fluorotec-F, Pfizer Inc., New York, New York) designed for rapid identification of members of the Bacteroides fragilis group (BFG) was evaluated. Tested were 228 clinical specimens (144 direct smears of clinical material, 14 smears of positive blood cultures, and 70 smears of colonies isolated from clinical material) and 49 reference strains of anaerobic bacteria, including 23 members of the BFG. Fluorotec-F detected 68 of 69 (98.5%) members of the BFG, including 55 B. fragilis, 12 B. thetaiotaomicron, and two B. ovatus, identified by cultural methods in all clinical specimens. Three specimens that yielded B. uniformis also fluoresced. Three specimens fluoresced but failed to yield members of the BFG or B. uniformis on culture. Of the 49 reference strains tested, all strains of B. fragilis, B. thetaiotaomicron, nd B. uniformis tested were detected by Fluorotec-F, but only five of a total of 14B. vulgatus, B. distasonis, and B. ovatus tested fluoresced. Of the 25 reference strains of anaerobic bacteria not belonging to the BFG, none fluoresced except for two strains of B. eggerthii. Direct fluorescent antibody staining of smears of clinical specimens suitable for anaerobic culture is a valuable tool for rapid detection of B. fragilis infections.

(PDF same-day service: $19.90)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 005395067

PMID: 7020409

DOI: 10.1093/ajcp/76.1.78

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Sheta, H. M.; Rubio, L.; Grafton Cardwell, E.; Polek, M.; Gumpf, D. J.; Falk, B. W.; Ullman, D. E., 2001: Is aphid transmission of Citrus tristeza virus a genetic bottleneck?. The effect of aphid and graft transmission on Citrus tristeza virus (CTV) quasispecies was studied by single strand conformation polymorphism (SSCP) and nucleotide sequence analysis. Sixteen CTV isolates were collected by aphid and graft transmiss...

Hurwitz, L.M.; Reiman, R.E.; Yoshizumi, T.T.; Goodman, P.C.; Toncheva, G.; Nguyen, G.; Lowry, C., 2007: Radiation dose from contemporary cardiothoracic multidetector CT protocols with an anthropomorphic female phantom: implications for cancer induction. Purpose: To measure prospectively and directly both organ dose and effective dose (ED) for adult cardiac and pulmonary computed tomographic (CT) angiography by using current clinical protocols for 64-detector CT in an anthropomorphic female phanto...

Gilmour, M.A.; Cardenas, M.R.; Blaik, M.A.; Bahr, R.J.; McGinnis, J.F., 2006: Evaluation of a comparative pathogenesis between cancer-associated retinopathy in humans and sudden acquired retinal degeneration syndrome in dogs via diagnostic imaging and western blot analysis. Objective-To evaluate dogs with sudden acquired retinal degeneration syndrome (SARDS) for evidence of pituitary gland, adrenal gland, and pulmonary neoplasia and antiretinal antibodies and to evaluate dogs with neoplasia for antiretinal antibodies...

Brew, A. H., 1992: Towards the use of pathogenic micro-organisms in the control of some cocoa insect pests in Ghana. Among the topics discussed in this article are the effects of unidentified entomogenous fungi parasitizing the mealybug vectors of cacao swollen shoot badnavirus, and the use of Bacillus thuringiensis to control caterpillars which predispose pods...

Orrell, J.M.; Brett, S.J.; Ivanyi, J.; Coghill, G.; Grant, A.; Beck, J.S., 1992: Measurement of the immunoperoxidase staining of macrophages within liver granulomata of mice infected with Mycobacterium tuberculosis. A quantitative image analysis technique developed for the measurement of the extent of macrophage activation and epithelioid cell differentiation was performed on mice infected experimentally with Mycobacterium tuberculosis. The granulomatous infl...

Lingeman, Carolyn, H., 1996: Trends in animal use in U.S. biomedical laboratories. The purpose of the study was to review U.S. laboratory animal use in the decades following enactment of the 1966 Animal Welfare Act and to evaluate trends in terms of reduction and replacement alternatives. Methods: Statistics on regulated species...

Fujimoto, Kenshiro; Kimoto, Hiromi; Shishikura, Mieko; Endo, Yasushi; Ogimoto, Keiji, 1993: Biohydrogenation of linoleic acid by anaerobic bacteria isolated from rumen. Bioscience Biotechnology & Biochemistry 57(6): 1026-1027

Cotrufo, M. F.; Ineson, P.; Rowland, A. P., 1994: Decomposition of tree leaf litters grown under elevated CO-2: Effect of litter quality. Ash (Fraxinus excelsior L.), birch (Betula pubescens Ehrh.), sycamore (Acer pseudoplatanus L.) and Sitka spruce (Picea sitchensis (Bong.) Carr.) leaf litters were monitored for decomposition rates and nutrient release in a laboratory microcosm exp...

Pascoal, Istenio F.; Umans, Jason G., 1995: Mechanisms of endothelium-dependent relaxation in human omental microvessels. Hypertension (Dallas) 26(3): 568

Bors, G.; Popa, I.; Voicu, Angela, 1967: Applications of paper chromatography and electro-phoresis to the isolation of quinine in forensic chemistry. The separation of quinine from the solvent extracts of cadaveric organs can be effected by chromatographic or electrophoretic methods after its transformation into its hydrochloride. In the chromatographic procedure a Rf value of 0.88 is obtained...