EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Evaluation of a direct fluorescent antibody staining method for rapid identification of members of the bacteroides fragilis group


American Journal of Clinical Pathology 76(1): 78-82
Evaluation of a direct fluorescent antibody staining method for rapid identification of members of the bacteroides fragilis group
A direct fluorescent antibody test kit (Fluorotec-F, Pfizer Inc., New York, New York) designed for rapid identification of members of the Bacteroides fragilis group (BFG) was evaluated. Tested were 228 clinical specimens (144 direct smears of clinical material, 14 smears of positive blood cultures, and 70 smears of colonies isolated from clinical material) and 49 reference strains of anaerobic bacteria, including 23 members of the BFG. Fluorotec-F detected 68 of 69 (98.5%) members of the BFG, including 55 B. fragilis, 12 B. thetaiotaomicron, and two B. ovatus, identified by cultural methods in all clinical specimens. Three specimens that yielded B. uniformis also fluoresced. Three specimens fluoresced but failed to yield members of the BFG or B. uniformis on culture. Of the 49 reference strains tested, all strains of B. fragilis, B. thetaiotaomicron, nd B. uniformis tested were detected by Fluorotec-F, but only five of a total of 14B. vulgatus, B. distasonis, and B. ovatus tested fluoresced. Of the 25 reference strains of anaerobic bacteria not belonging to the BFG, none fluoresced except for two strains of B. eggerthii. Direct fluorescent antibody staining of smears of clinical specimens suitable for anaerobic culture is a valuable tool for rapid detection of B. fragilis infections.


Accession: 005395067

PMID: 7020409

DOI: 10.1093/ajcp/76.1.78



Related references

Evaluation of a direct fluorescent antibody staining method for rapid herzfeld identification of members of the bacteroides fragilis group. Annals of Clinical & Laboratory Science 10(5): 441, 1980

Fluorescent antibody test kit for rapid detection and identification of members of the Bacteroides fragilis and Bacteroides melaninogenicus groups in clinical specimens. Journal of Clinical Microbiology 10(2): 121-127, 1979

Evaluation of fluorescent antibody methods for rapid identification of bacteroides fragilis. Abstracts of the Annual Meeting of the American Society for Microbiology 80: ABSTRACT 758, 1980

Evaluation of a fluorescent antibody technique for the rapid enumeration of Bacteroides fragilis group of organisms in water. Journal of Applied Bacteriology 62(4): 377-383, 1987

Fluorescent antibody techniques for the identification of group d streptococci direct staining method. Applied Microbiology 23(3): 571-577, 1972

Fluorescent-antibody techniques for the identification of group D streptococci: direct staining method. Applied Microbiology 23(3): 571-577, 1972

Evaluation of a rapid identification method for clinical isolates or bacteroides fragilis and other bacteroides using bacteriophages bile discs and bacteriocin. Abstracts of the Annual Meeting of the American Society for Microbiology 85: 325, 1985

Evaluation of the Rapid ID 32A system for the identification of the Bacteroides fragilis group. Clinical Microbiology and Infection 2(2): 115-122, 1996

Characterization of a polyvalent conjugate of bacteroides fragilis by fluorescent antibody staining. American Journal of Clinical Pathology 71(1): 97-101, 1979

Evaluation of the Rapid ID 32A system for identification of anaerobic Gram-negative bacilli, excluding the Bacteroides fragilis group. Clinical Microbiology and Infection 5(6): 319-326, 2002