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Evaluation of a fluorescent method in the study of the viability of candida albicans strains


, : Evaluation of a fluorescent method in the study of the viability of candida albicans strains. Revista de Microbiologia 18(3): 258-263

The effectiveness of the fluorescent viability test (fluorescein diacetate FDA and ethidium bromide-EB-solution) compared to the plaque counting test (Miles and Misra M&M) was performed on 10 samples of Candida albicans cultivated in Sabouraud dextrose agar at 25.degree. C. An ideal incubation period of 45 minutes was found. Growth curves of the fungi strains studied based on the mean cell number were drawn for both the FDA and EB and M&M methods. The statistical evaluation (Student's T test) of the average sum of the viable cell counts showed that the FDA-EB method is more sensitive than the M&M test for the studied species. The growth curves of the samples usually followed a homogeneous pattern comparable to others provenient from non-dimorphic fungi.

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