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Evaluation of a fluorescent method in the study of the viability of candida albicans strains


, : Evaluation of a fluorescent method in the study of the viability of candida albicans strains. Revista de Microbiologia 18(3): 258-263

The effectiveness of the fluorescent viability test (fluorescein diacetate FDA and ethidium bromide-EB-solution) compared to the plaque counting test (Miles and Misra M&M) was performed on 10 samples of Candida albicans cultivated in Sabouraud dextrose agar at 25.degree. C. An ideal incubation period of 45 minutes was found. Growth curves of the fungi strains studied based on the mean cell number were drawn for both the FDA and EB and M&M methods. The statistical evaluation (Student's T test) of the average sum of the viable cell counts showed that the FDA-EB method is more sensitive than the M&M test for the studied species. The growth curves of the samples usually followed a homogeneous pattern comparable to others provenient from non-dimorphic fungi.

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Kawashima Y.; Hirose A.; Kozuka H., 1984: Selective increase in acylation of 1 acylglycerophosphoryl choline in livers of rats and mice by peroxisome proliferators. Rats were fed a diet containing clofibric acid. Activity of microsomal 1-acylglycerophosphorylcholine (1-acyl-GPC) acyltransferase in liver was increased .apprx. 3-fold by the treatment with clofibric acid. The treatment of rats with clofibric aci...

Gittleson, B; Saltmarch, M; Cocotas, P; Mcproud, L., 1992: Quantification of the physical, chemical, and sensory modes of deterioration in sous-vide processed salmon. Commercially processed sous-vide salmon was stored between OC and 4C for 12 weeks to quantify various possible physical, chemical, and sensory modes of deterioration. Two types of packages were studied: (1) a two-web coextrusion using nylon/ethyl...

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Kolesova D.A., 1972: Ecology of pear aphids of the genus anuraphis in the crimea. Vestnik Zoologii 6(3): 11-15

Fomin, V. M.; Koshelenko, A. S.; Zhed' , O. V., 2005: Stress distribution in the bottom of the cylinder heads of diesel engines. A detailed study is made of the distribution of the temperature stresses between the valves (generally deemed to be the most vulnerable zone at the bottom of the cylinder head). Stress distributions are shown as isochrome charts, and the results o...

Clench, W. J., 1948: Two new genera and a new species of Syneeridae from the Caroline Islands. Occasional Papers of the Bishop Museum Honolulu, 198: 191-104

Keltner, Dacher, 2003: Expression and the course of life Studies of emotion, personality, and psychopathology from a social-functional perspective. Ekman, Paul [Editor, Reprint Author], Campos, Joseph J , Davidson, Richard J , de Waal, Frans B M Emotions inside out: 130 Years after Darwin's the expression of the emotions in man and animals : 222-243

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Vasconcelos, Heraldo L., 2002: Spatio-temporal variation in the foraging activity of the leaf-cutting ant Atta laevigata Variacao espaco-temporal na atividade forrageira da sauva Atta laevigata. Acta Amazonica. Janeiro-Marco; 321: 141-154