+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Evaluation of a fully mechanized enzymatic kinetic determination of sialic acid

Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift für Klinische Chemie und Klinische Biochemie 23(11): 781-785

Evaluation of a fully mechanized enzymatic kinetic determination of sialic acid

The enzymatic determination of sialic acid, commercially introduced recently as an end point method, has been considerably improved by the development of initial rate and peak rate modifications of the kinetic method, respectively, and adapted to a centrifugal analyser (Cobas Bio). The assay is completed within 3 min, takes 3 microliter sample volume and is reduced to a total reaction volume of 153 microliter. The costs per test thereby are lowered to about 10% of the original procedure. Within-run and day-to-day imprecisions (means = 0.81 g/l) are 2% and 3.9%, respectively; inaccuracy is between +1% and -2%. The detection limit is 0.012 g/l, i.e. about 3 times higher than that of the thiobarbituric acid method (0.0037 g/l) for sialic acid determination. The enzymatic-kinetic assay correlates well with the latter method (r = 0.973). There was no measurable interference by EDTA (less than 3 mg/l), bilirubin (less than 160 mumol/l), haemolysis (haemoglobin less than 5.25 g/l), or triglycerides (less than 3.50 g/l). The reference range (2.5th to 97.5th percentile) for sialic acid in sera of adults is 0.51 to 0.84 g/l (median 0.63 g/l) with no significant sex-related differences. The reference range for sialic acid in spontaneous urine samples is 0.01-0.13 g/l.

(PDF same-day service: $19.90)

Accession: 005395121

PMID: 4086982

DOI: 10.1515/cclm.1985.23.11.781

Related references

Enzymatic determination of serum uric acid by means of fully mechanized analysis systems. Das Medizinische Laboratorium 30(2): 46-56, 1977

A simple enzymatic determination of urinary sialic acid--its significance in the diagnosis of disorders of sialic acid metabolism. Clinica Chimica Acta; International Journal of Clinical Chemistry 144(2-3): 263-267, 1984

Evaluation of a colorimetric enzymatic method for the determination of sialic acid in serum. Quaderni Sclavo di Diagnostica Clinica E di Laboratorio 23(1): 18-23, 1987

Evaluation of an automated enzymatic method for the determination of serum sialic acid. Proceedings of the American Association for Cancer Research Annual Meeting 28: 183, 1987

Evaluation And Modification Of Sialic Acid Determination Methods--Sialic Acid Determination In Various Mammalian Tissues As Well As In Various Tumor Cells. Seikagaku. Journal of Japanese Biochemical Society 36: 806-815, 1964

Kinetic determination of alpha amylase in serum and urine with an oligo saccharide as substrate modification for fully mechanized enzyme measuring device. Journal of Clinical Chemistry & Clinical Biochemistry 17(11): 705-708, 1979

A re-evaluation of plasma sialic acid determination using the periodate resorcinol method versus the enzymatic method. Southeast Asian Journal of Tropical Medicine and Public Health 36(4): 1011-1013, 2005

New enzymatic determination of sialic acid in serum. Clinical Chemistry 34(11): 2291-2294, 1988

Fully mechanized kinetic measurement of creatinine. Das Medizinische Laboratorium 29(6): 137-145, 1976

Multicenter evaluation of a fully mechanized soluble transferrin receptor assay on the Hitachi and cobas integra analyzers. the determination of reference ranges. Clinical Chemistry and Laboratory Medicine 40(5): 529-536, 2002